油茶炭疽病拮抗细菌P-14的拮抗物质分析

Analysis of Metabolic Antagonists Against Colletotrichum camellia by Antagonistic Bacteria P-14

[目的]探索解淀粉芽孢杆菌P-14对油茶炭疽病病原菌的拮抗物质,以期为油茶炭疽病的生物农药开发利用提供理论依据。[方法]利用酸沉淀法和高效液相色谱法分离解淀粉芽孢杆菌P-14发酵液中的拮抗物质,通过顶空固相微萃取法和气相色谱-质谱法对解淀粉芽孢杆菌P-14的挥发性物质进行了分离鉴定。[结果]分离后的组分a对油茶炭疽病的病原菌具有良好的抑制作用,其主要成分为C15 bacillomycin D(杆菌霉素D)。此外,共分离得到35种挥发性化合物,其相对抑菌率为(26.19±3.82)%,其中包含苯类物质(benzenes)10种、烷基类物质(alkyls)8种、醇类物质(alcohols)2种、酮类物质(ketones)11种、醛类物质(aldehydes)3种和1种酯类物质(esters)。[结论]解淀粉芽孢杆菌P-14能产生对油茶炭疽病的病原菌具有明显的拮抗作用的物质,并确定了其拮抗物质种类,为油茶炭疽病的生物农药开发提供了可靠的研究内容。

[Objective] The purpose of this study is to explore the metabolic antagonists against Colletotrichum camellia produced by antagonistic bacteria P-14, in order to provide a theoretical basis for the development and utilization of biopesticides of Camellia oleifera.[Method] The acid precipitation and high-performance liquid chromatography was used to identify the lipopeptide antibiotics produced by strain P-14, and the volatile compounds were analyzed by gas chromatography-mass spectrometry.[Result] The component a containing C15 bacillomycin D showed the strongest inhibitory effect against Colletotrichum camellia. In addition, a total of 35 volatile compounds were separated, the relative bacteriostatic rate was (26.19±3.82)%, including 10 benzenes, 8 alkyls, 2 alcohols, 11 ketones, 3 aldehydes, and 1esters.[Conclusion] Bacillus amyloliquefaciens P-14 could produce metabolic antagonists against Colletotrichum camellia, and the metabolic antagonists has been identified, which can provide with reliable research data for the development of biopesticides against C. oleifera.