MicroRNA-301a在胃肠间质瘤中的表达差异与功能

Altered expression profile and function of microRNA-301a in gastric stromal tumor

目的筛选胃肠间质瘤的microRNAs(miRNAs)表达谱差异并探讨microRNA-301a(miR-301a)的功能。方法收集45例原发胃肠间质瘤患者的肿瘤及癌旁正常组织,通过miRNA芯片技术获得其中5例患者的肿瘤miRNA表达谱,并采用荧光定量PCR技术检测其余40例患者异常的miRNA表达水平作为验证实验。将显著上调的miR-301a表达量与患者临床病理特征行相关性分析,并采用MTT实验探索miR-301a对胃肠间质瘤细胞系生长的影响。结果在胃肠间质瘤组织中筛选出5种高表达的microR-NA和5种低表达的microRNA,其中miR-301a的表达上调最为明显。其表达量与肿瘤的危险度分级、肿瘤大小、肿瘤细胞核分裂象和有无坏死密切相关(P <0.05),在胃肠间质瘤细胞系中过表达miR-301a能显著增强细胞的增殖能力。结论 miR-301a在胃肠间质瘤组织中显著上调,与恶性临床病理特征密切相关并能影响体外肿瘤细胞的生长增殖,miR-301a可能是未来胃肠间质瘤治疗的潜在靶点。

Objective The aim was to screen the expression profiles of microRNAs(miRNAs)in gastrointestinal stromal tumors(GIST)and explore the function of microRNA-301a(miR-301a). Methods Collected the tumor and adjacent normal tissues of 45 patients,who were diagnosed primary GIST. The expression profiles of tumor miRNAs in 5 of the 45 patients were obtained by microarray technology,and the abnormal expression levels of miRNAs in the remaining 40 patients were detected by Real Time-PCR as a validation experiment. Correlation analysis was analyzed between the significantly up-regulated expression of miR-301a and the clinicopathological features of the patients. The MTT experiment was used to explore the effect of miR-301a on the growth of GIST cell lines. Results Five kinds of miRNAs with high expression and five kinds of miRNAs with low expression were screened out from GIST,of which the expression of miR-301a was up-regulated most obviously. The expression of miR-301a was closely related to tumor risk grade,tumor size,mitosis and necrosis(P < 0.05). The overexpression of miR-301a in GIST cell lines could significantly enhance the proliferation of cells. Conclusions MiR-301a was up-regulated in GIST,which was closely related to malignant clinicopathological features and could affect the growth and proliferation of tumor cells in vitro. MiR-301a might be a potential target for future treatment of GIST.