SIAH2在卵巢上皮性癌中的表达及意义

Expression and significance of SIAH2 in epithelial ovarian carcinoma

目的研究E3泛素连接酶SIAH2在卵巢上皮性癌组织中的表达及临床意义,探讨其在卵巢上皮性癌发生、发展中的作用。方法应用免疫组织化学染色检测165例卵巢组织标本SIAH2的表达,用Western blot法检测卵巢新鲜组织中SIAH2的表达,结合患者随访资料,利用Kaplan-Meier法绘制生存曲线,Log-rank法分析SIAH2的表达水平与卵巢上皮性癌患者预后的关系,Cox比例风险回归模型分析卵巢上皮性癌患者预后的独立预测因素。结果 SIAH2在卵巢上皮性癌中阳性表达率为76.4%,显著高于卵巢交界性肿瘤(41.7%)、卵巢良性囊肿(5.13%)和正常卵巢组织(2.86%,均P <0.05)。而SIAH2在卵巢良性囊肿和正常卵巢组织中的表达差异无统计学意义。SIAH2的表达与组织学分级、FIGO分期及淋巴结转移显著相关(均P <0.05);在正常卵巢组织、卵巢良性囊肿、卵巢交界性肿瘤及卵巢上皮性癌中SIAH2的相对表达量分别是0.12±0.05、0.11±0.04、0.57±0.08、1.05±0.10;正常卵巢组织和卵巢良性囊肿SIAH2的表达差异无统计学意义,从卵巢组织(卵巢良性囊肿)到卵巢交界性肿瘤再到卵巢上皮性癌组织中SIAH2的表达依次升高,差异均有统计学意义(均P <0.05);SIAH2(+)与SIAH2(-)患者的生存曲线差异有统计学意义(P <0.05);多因素回归分析表明高表达的SIAH2是卵巢上皮性癌患者总生存期的独立预测因子。结论过表达的SIAH2可能在卵巢上皮性癌发生和进展中发挥着重要作用,SIAH2可作为生物标志物预测卵巢上皮性癌患者的不良预后。

Objective To analyze the expression and clinicopathological significance of seven in absentia homolog 2(SIAH2)in epithelial ovarian carcinoma,and to discuss its role in the development of epithelial ovarian carcinoma. Methods The expression of SIAH2 was determined by immunohistochemical S-P method in 165 cases of ovarian samples and SIAH2 expression was examined by Western blot. In the combination with follow-up data, survival curves were calculated using the Kaplan-Meier method and relationship between SIAH2 expression and prognosis of ovarian carcinoma patients was analyzed by the log-rank test. Cox proportional hazards regression model was used to examine the independent predictive factors in the patients with epithelial ovarian carcinoma. Results SIAH2 expression in epithelial ovarian cancer(76.4%)was higher than that of borderline ovarian tumors (41.7%),benign ovarian cysts(5.13%)and normal ovarian tissues(2.86%),and there were significant differences (all P < 0.05). No statistical significance of SIAH2 expression was found between benign ovarian cysts and normal ovarian tissues(P > 0.05). The expression of the SIAH2 was significantly correlated to histological grade,FIGO stage and lymph node metastasis(P < 0.05). The relative expression of SIAH2 in normal ovarian tissue,benign ovarian cyst,borderline ovarian tumor and epithelial ovarian cancer was 0.12 ± 0.05,0.11 ± 0.04,0.57 ± 0.08 and 1.05 ± 0.10,respectively. No difference of SIAH2 expression was found between normal ovarian tissues and benign ovarian cysts(P > 0.05). The expression of SIAH2 increased from ovarian tissues / benign ovarian cysts to borderline ovarian tumor to epithelial ovarian cancer and the difference was statistically significant(all P < 0.05). The survival curves of patients with SIAH2(+)differed from those of patients with SIAH2(-)and the difference was statistically significant(P < 0.05). Multiple factor analysis revealed that the higher expression of SIAH2 was an independent prognostic factor for overall survival. Conclusions The over-expression of SIAH2 plays an important role in the tumorigenesis and progression of epithelial ovarian cancer. The over-expression of SIAH2 may serve as a biomarker for poor prognosis of epithelial ovarian cancer patients.