枯草芽孢杆菌CGMCC 6624对抗生素耐受性的研究

Study on the Resistance of Bacillus subtilis CGMCC 6624 to Atibiotics

采用双层牛津杯法研究枯草芽孢杆菌CGMCC 6624对抗生素的耐受性。结果显示:当抗生素浓度在1×10-5～1mg/mL时,枯草芽孢杆菌CGMCC 6624对硝基咪唑类中的替硝唑具有耐受性;当抗生素浓度为1×10-3～1mg/mL时,枯草芽孢杆菌CGMCC 6624对四环素类中的四环素和土霉素,喹诺酮类中的氧氟沙星具有耐受性;当抗生素浓度在1×10-2～1mg/mL时,枯草芽孢杆菌CGMCC 6624对β-内酰胺类中的阿莫西林、青霉素和氨苄青霉素,氨基糖苷类中的链霉素,酰胺醇类中的氯霉素、甲砜霉素,大环内酯类中的红霉素和硝基咪唑类中的甲硝唑具有耐受性;当抗生素浓度在1×10-1～1mg/mL时,枯草芽孢杆菌CGMCC 6624对氨基糖苷类中的庆大霉素、新霉素,酰胺类中的氟苯尼考和磺胺类中的磺胺具有耐受性;当抗生素浓度为1mg/mL时,枯草芽孢杆菌CGMCC 6624对大环内酯类中的阿奇霉素具有耐受性。以上结果表明枯草芽孢杆菌CGMCC 6624对不同种类的抗生素具有一定的耐受性。该研究为该菌株在食品、饲料中的应用奠定了理论基础。

The resistance of Bacillus subtilis CGMCC 6624 to antibiotics is researched using double-layered Oxford cup method.The results show that B.subtilis CGMCC 6624 is resistant to tinidazole in nitroimidazole when the antibiotic concentration is1×10^-5~1 mg/mL;B.subtilis CGMCC 6624 is resistant to tetracycline and oxytetracycline in tetracycline,and ofloxacin in quinolones when the antibiotic concentration is1×10^-3~1 mg/mL;B.subtilis CGMCC 6624 is resistant to amoxicillin,penicillin and ampicillin inβ-lactams,streptomycin in aminoglycosides,chloramphenicol,thiamphenicols in amide alcohols,erythromycin in macrolides and metronidazole in nitroimidazoles;B.subtilis CGMCC 6624 is resistant to gentamicin,neomycin in aminoglycosides,florfenicol in amide and sulfonamide in sulfonamides when the antibiotic concentration is1×10^-1~1 mg/mL;B.subtilis CGMCC 6624 is resistant to azithromycin in macrolides when the antibiotic concentration is1 mg/mL.The above results indicate that B.subtilis CGMCC 6624 is resistant to different types of antibiotics.This research has laid a theoretical foundation for the application of strains in food and feed.