草莓脱毒组培苗低成本快繁技术研究

Study on the Tissue Culture and Rapid Propagation with Low Cost of Strawberry(Fragaia ananassa Duchesne) Virus-free Seedling

为降低草莓脱毒组培苗的生产成本,从碳源种类及浓度、凝固剂种类及浓度、培养容器等方面进行了低成本快速繁殖技术的研究。结果表明,蔗糖占到总成本的52.5%,凝固剂占到总成本的38.9%,用白砂糖替换蔗糖、卡拉胶替换琼脂粉,总成本降为原来的32.5%,大大节约了成本;采用带有塑料瓶盖的玻璃瓶,可节约劳动力,有效地降低了成本,从而极大地提高了生产效率。

To reduce production costs of strawberry virus-free seedling,the process of tissue culture about carbon source,gelling agent and culture container was studied.The result showed that the cost of sucrose and coagulant was higher,which accounted for 52.5% and 38.9% of the total cost,respectively.It could reduce the cost to 32.5% by sucrose replaced by white sugara and agar replaced by gel as gelling agent.Used with cover breathable plastic tube instead of glass flask as culture container,reduce the cost by saving labor,thus greatly improve the production efficiency.