瑞舒伐他汀和阿司匹林对兔腹主动脉粥样硬化斑块疗效的磁共振研究

Effect of Rosuvastatin and Aspirin therapy for abdominal aortic atherosclerotic plaque in rabbits :a magnetic resonance imaging study

目的应用磁共振成像评价瑞舒伐他汀、阿司匹林以及二者联合用药对兔动脉粥样硬化斑块的疗效,并探讨其对炎症细胞的抑制作用。方法 160只新西兰兔行高脂饮食联合腹主动脉内膜损伤术,诱导动脉粥样硬化斑块的发生。所有动物随机分成对照组(高脂饮食)、A组(高脂饮食+阿司匹林)、T组(高脂饮食+瑞舒伐他汀)和AT组(高脂饮食+阿司匹林+瑞舒伐他汀),每组各40只;每组动物又随机分成1周组、2周组、1个月组和2个月组,每组各10只。分别于相应时间点行腹主动脉MR检查,分析斑块负荷即管壁标准化指数(NWI)的变化。MR检查后立即处死动物,尸检取得腹主动脉标本,行HE染色和巨噬细胞免疫组化染色,分析腹主动脉斑块形态学、斑块巨噬细胞含量的变化。结果 A组、T组和AT组NWI在各时间点均较对照组减低(1周、1个月及2个月时,P<0.05)。随时间延长对照组动脉内膜逐渐增厚;在各时间点A组、T组和AT组动脉内膜增厚程度均较对照组减轻。在2个月时,A组、T组和AT组的巨噬细胞含量较对照组减少。结论瑞舒伐他汀、阿司匹林和两药联合均能抑制斑块进展,减轻斑块内炎症反应;高分辨MRI可用于对药物治疗效果的评估。

Objective To evaluate therapeutic effect of rosuvastatin,aspirin and two-drug combination on atherosclerotic plaque by conventional magnetic resonance imaging,explore its inhibition of inflammatory cells.Methods 160 New Zealand rabbits were given endothelial denudation combined with a cholesterol-enriched diet to induce abdominal aortic atherosclerosis.all rabbits were assigned randomly to control group(high fat diet),group A(high fat diet with aspirin),group T(high fat diet with rosuvastatin)and group AT(high fat diet with aspirin as well as rosuvastatin),40 for each group.Then animals were divided randomly into 1 week,2 weeks,1 month and 2 months subgroup,10 for each subgroup.Abdominal aortic MR examinations were performed to analyze the AS plaque load,and wall normalization index(NWI).Abdominal aortic specimens were stained with HE and macrophage immunohistochemical staining to analyze plaque morphology and macrophage content.Results The NWI of A,T and AT group were lower than the control group at each time point(1 week,1 month and 2 months,P<0.05).In control group,the arterial intima gradually thickened over time.At each time point,the degree of arterial intimal thickening in group A,T,AT were less than that in control group.After 2 months,the macrophages of plaque with group A,T,AT were all decreased compared with the control group.Conclusion Rosuvastatin,aspirin and two drug combination can restrain plaque load,reduce inflammatory response.MR can be used in the evaluation of drug therapy.