一种新型H_2O_2近红外荧光探针的制备及其应用

A Near-infrared Fluorescent Probe for Hydrogen peroxide Base on IR-780 as Fluorophore

本文结合了近红外荧光与去保护机理H2O2荧光探针的优点,设计了一种H2O2的近红外荧光探针。该探针以三碳菁染料IR-780为荧光底物,其激发和发射波长均位于近红外区内,并在三碳菁染料IR-780上结合硼醚作为H2O2的感应机团,以实现H2O2的选择性测定。并将该产物用于H2O2的检测,研究其在H2O2水溶液中的荧光的响应特性,并且证明该物质的非氧化荧光增强机理。

In this report,we presented a novel near-infrared fluorescent probe for H2O2 .IR-780 is used as a fluorophore,whose excitation and emission wavelengths are in near-infrared region,and are less absorbed by molecules and can penetrate more deeply into tissues and minimizes cell damage and autofluorescent by excitation light.The new probe are highly sensitive to H2O2 with detection limit of 5.2×10^-11 mol·L^-1 and well selectively over competing cellular ROS such as superoxide(O2^-),nitric oxide(NO),and lipid peroxides.