马齿苋-肉桂联用提取工艺及其对DSS诱导溃疡性结肠炎的作用研究

Study on Extraction Process of Portulaca oleracea Combining with Cinnamomum cassia and Its Effect on Ulcerative Colitis Induced by DSS

目的:对马齿苋-肉桂联用最佳配比和多糖提取工艺及其多糖抗实验性溃疡性结肠炎(UC)作用进行研究。方法:采用L_9(3~4)正交试验对马齿苋-肉桂联用最佳配比以及多糖的提取工艺进行优化;用3%葡聚糖硫酸钠(DSS)诱导小鼠UC模型,灌胃300 mg·kg^(-1)柳氮磺胺吡啶为阳性对照,马齿苋-肉桂低中高实验组分别以100、200、400 mg·kg^(-1)的多糖水溶液灌胃治疗,观察小鼠给药前后的体征、结肠组织形态、血清白细胞介素-10(IL-10)及肿瘤坏死因子(TNF-α)的含量变化。结果:最佳提取工艺为马齿苋-肉桂12∶5,料液比1∶30,90℃提取5 h;给药治疗UC小鼠后,HE染色显示小鼠结肠黏膜上皮溃疡处有不同程度修复;给药组小鼠血清抑炎因子IL-10含量较模型组显著上升(P<0.01),促炎因子TNF-α含量较模型组显著降低(P<0.01)。结论:本实验得出马齿苋配伍肉桂联用最佳配比和最佳提取工艺。给药治疗UC小鼠后,从疾病活动指数(DAI)评分、HE染色、IL-10及TNF-α含量反映,马齿苋-肉桂联用对UC有治疗作用,为临床治疗UC提供了新思路。

Objective:To explore the optimal ratio of Portulaca oleracea combing with Cinnamomm cassia and evaluate their protecitive against DSS induced ulcerative colitis.Methods:Orthogonal experiment design L9(3^4)was employed to optimize the extraction process.UC mice were induced by 3%DSS and the DAI,H&E staining were enrolled to evaluated the protective effect of the extraction.The expression levels of IL-10 and TNF-αwere also detected.Results:The best ratio of P.oleracea combing with C.cassia was 12:5.The extraction process was verified at 90℃for 5 h.After administration of the extraction,the morphological structure of UC was improved and the inflammatory cells were almost disappeared,indicating the satisfied therapeutic effect.The expression level of IL-10 was greatly increased,while the pro-inflammatory cytokine TNF-αwas decreased significantly.Conclusion:P.oleracea combing with C.cassia could improve the health conditions of UC mice via regulating the levels of inflammatory cytokines.And the extraction also can improve the morphological structure of UC.