摘要
目的:对马齿苋-肉桂联用最佳配比和多糖提取工艺及其多糖抗实验性溃疡性结肠炎(UC)作用进行研究。方法:采用L_9(3~4)正交试验对马齿苋-肉桂联用最佳配比以及多糖的提取工艺进行优化;用3%葡聚糖硫酸钠(DSS)诱导小鼠UC模型,灌胃300 mg·kg^(-1)柳氮磺胺吡啶为阳性对照,马齿苋-肉桂低中高实验组分别以100、200、400 mg·kg^(-1)的多糖水溶液灌胃治疗,观察小鼠给药前后的体征、结肠组织形态、血清白细胞介素-10(IL-10)及肿瘤坏死因子(TNF-α)的含量变化。结果:最佳提取工艺为马齿苋-肉桂12∶5,料液比1∶30,90℃提取5 h;给药治疗UC小鼠后,HE染色显示小鼠结肠黏膜上皮溃疡处有不同程度修复;给药组小鼠血清抑炎因子IL-10含量较模型组显著上升(P<0.01),促炎因子TNF-α含量较模型组显著降低(P<0.01)。结论:本实验得出马齿苋配伍肉桂联用最佳配比和最佳提取工艺。给药治疗UC小鼠后,从疾病活动指数(DAI)评分、HE染色、IL-10及TNF-α含量反映,马齿苋-肉桂联用对UC有治疗作用,为临床治疗UC提供了新思路。
Objective:To explore the optimal ratio of Portulaca oleracea combing with Cinnamomm cassia and evaluate their protecitive against DSS induced ulcerative colitis.Methods:Orthogonal experiment design L9(3^4)was employed to optimize the extraction process.UC mice were induced by 3%DSS and the DAI,H&E staining were enrolled to evaluated the protective effect of the extraction.The expression levels of IL-10 and TNF-αwere also detected.Results:The best ratio of P.oleracea combing with C.cassia was 12:5.The extraction process was verified at 90℃for 5 h.After administration of the extraction,the morphological structure of UC was improved and the inflammatory cells were almost disappeared,indicating the satisfied therapeutic effect.The expression level of IL-10 was greatly increased,while the pro-inflammatory cytokine TNF-αwas decreased significantly.Conclusion:P.oleracea combing with C.cassia could improve the health conditions of UC mice via regulating the levels of inflammatory cytokines.And the extraction also can improve the morphological structure of UC.
作者
任浪浪
唐志书
梁艳妮
刘力
宋忠兴
黄文静
王征
REN Lang-lang;TANG Zhi-shu;LIANG Yan-ni;LIU Li;SONG Zhong-xing;HUANG Wen-Jing;WANG Zheng(Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Shaanxi Province Key Laboratory of New Drugs and Chinese Medicine Foundation Research,Shaanxi Rheumatism and Tumor Center of TCM Engineering Technology Research,Xianyang 712083,China)
出处
《中国现代中药》
CAS
2019年第2期182-187,198,共7页
Modern Chinese Medicine
基金
国家自然科学基金项目(81703924)
陕西省高校科协青年人才托举计划项目(20160229)
关键词
马齿苋
肉桂
联用
提取工艺
溃疡性结肠炎
Portulaca oleracea
Cinnamomum cassia
combing with
extraction process
ulcerative colit