阿魏酸诱导胃癌SGC-7901细胞凋亡及对COX-2、Survivin、XIAP和p53的影响

The Inducing Effects of Ferulic Acid on the Apoptosis of Gastric Cancer SGC-7901 Cells and Its Influence on COX-2, Survivin, XIAP and p53

目的:探讨阿魏酸对人胃癌SGC-7901细胞增殖的影响及其对凋亡相关基因表达的影响。方法:不同浓度(0、1.25、2.5、5、7.5、10、12.5 mg/mL)阿魏酸作用体外培养人胃癌SGC-7901细胞24、48、72小时后,MTT法检测细胞增殖;不同浓度(0、5、7.5、10 mg/mL)阿魏酸作用SGC-7901细胞48小时,流式细胞仪检测细胞凋亡,RT-q PCR和免疫印迹法(Western-blot)检测环氧化酶2(COX-2)、生存素(Survivin)、X连锁凋亡抑制蛋白(XIAP)和p53的mRNA和蛋白表达。结果:与对照组比较,7.5、10、12.5 mg/mL阿魏酸使SGC-7901细胞的OD值降低,5、7.5、10 mg/mL阿魏酸作用SGC-7901细胞48小时后均能诱导其凋亡,5、7.5、10 mg/mL阿魏酸均能上调p53的mRNA和蛋白表达,下调COX-2、Survivin和XIAP的mRNA和蛋白表达。结论:阿魏酸能有效抑制SGC-7901细胞增殖,并能诱导其凋亡,阿魏酸能上调胃癌细胞p53的mRNA和蛋白表达,下调COX-2、Survivin和XIAP的mRNA和蛋白表达,发挥抗肿瘤作用。

Objective:To discuss the effects of ferulic acid on the proliferation of human gastric cancer SGC-7901 cells and its influence on the expression of apoptosis-related gene.Methods:Different concentrations of ferulic acids were used to intervene human gastric cancer SGC-7901 cells cultured in vitro,after acting on for 24,48 and 72 hours,MTT method was used to detect cellular proliferation;after different concentrations(0,5,7.5 and 10 mg/mL)of ferulic acids acting on SGC-7901 cells for 48 hours,flow cytometry was adopted to detect cellular apoptosis,RT-q PCR and Western-blot were uased to measure mRNA and protein expressions of COX-2,Survivin,XIAP and p53.Results:Compared with the control group,ferulic acids in the concentrations of 7.5,10 and 12.5 mg/mL could decrease OD values of SGC-7901 cells,ferulic acids in the concentrations of 5,7.5 and 10 mg/mL after acting on SGC-7901 cells for 48 hours could induce cellular apoptosis,ferulic acids in the concentrations of 5,7.5 and 10 mg/mL could increase mRNA and protein expression of p53,decrease mRNA and protein expressions of COX-2,Survivin and XIAP.Conclusion:Ferulic acid could effectively inhibit the proliferation of SGC-7901 cells,induce its apoptosis,it could increase mRNA and protein expression of gastric cancer p53,decrease mRNA and protein expression of COX-2,Survivin and XIAP,and produce anti-tumor effects.