艾司西酞普兰对MPTP处理的PC12细胞的保护作用及机制

Protective effect and mechanism of estalopram on PC12 cells treated with MPTP

目的 :探讨艾司西酞普兰(ESC)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)处理的PC12细胞的保护作用及其机制。方法 :用不同浓度ESC或/和不同浓度MPTP处理PC12细胞,再用3-甲基腺嘌呤(3-MA)抑制;通过MTS检测细胞存活率,Hoechst 33258染色观察细胞凋亡,免疫印迹检测自噬标记物LC3B-Ⅱ、LC3B-Ⅰ和凋亡蛋白Bcl-2、Bax的表达。结果 :经筛选得出,10μmol/L ESC预处理PC12细胞1 h,再加入1 000μmol/L MPTP处理24 h,细胞存活率显著提高;Hoechst 33258染色显示细胞核固缩明显减少;ESC+MPTP组LC3B-Ⅱ/Ⅰ、Bcl-2/Bax表达水平较MPTP组显著上升,但ESC+MPTP+3MA组LC3B-Ⅱ/Ⅰ、Bcl-2/Bax表达水平均显著下降。结论 :ESC对MPTP处理的PC12细胞有一定的神经保护作用,可能通过PI3K/Akt途径激发自噬作用,提高了Bcl-2的表达,抑制MPTP引发的细胞凋亡,从而发挥对其保护作用。

Objective:To investigate the protective effect and mechanism of estalopram(ESC)on PC12 cells treated with 1-methyl-4-phenyl-6-tetrahydropyridine(MPTP).Methods:PC12 cells were treated with different concentrations of ESC or/and MPTP and then inhibited by 3-methyladenine(3-MA).The cell viability rate was detected by MTS.Cell apoptosis was detected by Hoechst 33258 staining.The expression of autophagy marker LC3B-Ⅱ,LC3B-Ⅰand apoptotic protein Bcl-2,Bax were detected by Western blotting.Results:PC12 cells were pretreated with 10μmol/L ESC for 1 h,and then treated with 1 000μmol/L MPTP for 24 h.The cell viability rate was significantly increased.Hoechst 33258 staining showed that nuclear pyknosis was significantly decreased.The expression of LC3B-Ⅱ/Ⅰand Bcl-2/Bax in ESC+MPTP group was significantly higher than that in MPTP group,but the expression of LC3B-Ⅱ/Ⅰand Bcl-2/Bax in ESC+MPTP+3MA group was significantly lower than that in MPTP group.Conclusion:ESC has a neuroprotective effect on PC12 cells treated with MPTP,which may stimulate autophagy through PI3K/Akt pathway,increase the expression of Bcl-2 and inhibit the apoptosis induced by MPTP.