反式-茴香脑对叠氮钠诱导的PC12细胞损伤的保护作用

Protective effect of trans-anethole on PC12 cell injury induced by sodium azide

目的探讨反式-茴香脑对叠氮钠诱导的大鼠肾上腺嗜铬细胞瘤细胞(PC12细胞)损伤的保护作用及作用机制。方法将叠氮钠(9～54 mmol·L^(-1))与PC12细胞共同孵育,采用CCK-8法检测细胞存活率;倒置显微镜观察细胞形态;Hoechst 33258染色法检测细胞凋亡;比色法测定细胞乳酸脱氢酶(LDH)漏出量、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活力。结果叠氮钠对PC12细胞的损伤呈剂量依赖性。反式-茴香脑与PC12细胞孵育48 h对细胞的存活率无影响;利用10、60μmol·L^(-1)反式-茴香脑预处理PC12细胞8 h,可明显对抗叠氮钠致神经细胞存活率下降,明显降低细胞LDH的漏出率和细胞MDA含量,明显提高细胞SOD活力。结论反式-茴香脑对叠氮钠诱导的PC12细胞损伤有明显的保护作用,其作用机制可能与提高PC12细胞的抗氧化酶活性有关。

Aim To investigate the protective effect of trans-anethole on the PC12 cell injury induced by sodium azide.Methods PC12 cells were exposed to sodium azide with concentration ranged from 9~54 mmol·L^-1.The survival rate of the cells was determined by CCK-8 assay.The cell morphology was detected with the inverted microscope;the apoptosis of cells was detected by Hoechst 33258 staining;the content of LDH,MDA levels and antioxidant enzyme activities of SOD were determined using the assay kits according to the manufacturer′s protocol.Results PC12 cell injury induced by sodium azide presented a dose-effect relationship.The PC12 cell viability was not changed when treated by trans-anethole for 48 h.Pretreatment of PC12 cells with 10μmol·L^-1 and 60μmol·L-1 trans-anethole for 8 h could obviously reduce cell injury,reduce LDH leakage and MDA level,as well as increase the antioxidant enzyme activities of SOD induced by sodium azide.Conclusions Trans-anethole exerts significant protective effect on PC12 cell injury induced by sodium azide.The protective mechanism might be executed by increasing the activities of antioxidant enzymes.