Urantide对动脉粥样硬化大鼠肾组织中STAT3表达的影响

Effect of Urantide on renal expression of STAT3 in atherosclerotic rats

目的探讨UⅡ受体拮抗剂——Urantide对动脉粥样硬化(AS)大鼠急性肾损伤(AKI)中信号传导与转录激活因子3(STAT3)表达的影响。方法采用经腹腔注射维生素D_3(Vit D_3)及饲以含高脂成分特制饲料的方法复制AS大鼠模型,按照随机原则分为4组:正常组、模型组、辛伐他汀组、Urantide组。采用HE染色观察各组大鼠肾小球形态结构变化;全自动生化分析仪检测大鼠血清中尿素氮(BUN)含量变化;Western blotting检测肾组织中STAT3、p-STAT3蛋白表达水平。结果模型组HE染色显示肾小管间质水肿,部分肾小球萎缩,肾小球囊壁与周围组织粘连;模型组大鼠血清中BUN含量及肾组织中STAT3、p-STAT3蛋白表达水平较正常组升高(P <0.05)。与模型组比较,辛伐他汀组大鼠肾小球和肾小管间质的形态学变化趋于正常组,大鼠血清中BUN含量降低(P <0.05),肾组织中STAT3、p-STAT3蛋白表达水平升高(P <0.05);Urantide组大鼠肾小球萎缩和肾小管间质水肿的形态学变化有所改善,大鼠血清中BUN含量降低(P <0.05),肾组织中STAT3、p-STAT3蛋白表达水平降低(P <0.05)。结论 Urantide可通过调节STAT3的表达缓解AKI,以及改善肾小球的功能。

Objective To investigate the effect of Urantide on renal expression of signal transduction and transcription activator 3(STAT3)in atherosclerotic(AS)rats.Methods The rat model of AS was established by an intraperitoneal injection of vitamin D3(Vit D3)plus high fat diet.Rats were randomly divided into normal group,AS group,Simvastatin group and Urantide group.HE staining was performed to observe the glomerular structure and morphological changes of kidney.Serum urea nitrogen(BUN)and expression levels of STAT3 and p-STAT3 protein in renal tissue were measured.Results HE staining showed the tubular interstitial edema,partial glomerular atrophy,adhesion of the glomerular cyst wall to the surrounding tissue in AS group.Expression of serum BUN,STAT3 and p-STAT3 in rat kidneys were enhanced in AS group compared with those in normal control group(P<0.05).Simvastatin group showed significant improvement in morphological damage of kidney,decreased levels of serum BUN(P<0.05)while increased expression of STAT3 and p-STAT3 when compared with those in AS group(P<0.05).Rats in Urantide group experienced significant improvement in morphological damage of kidney,decreased levels of serum BUN,STAT3 and p-STAT3 compared with those in AS group(P<0.05).Conclusions Urantide alleviates the acute kidney injury and improves the function of glomeruli by regulating the expression of STAT3.