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儿茶酚胺抑素在自发性高血压大鼠交感神经活化中的作用 被引量:7

Role of Catestatin in sympathetic activation in spontaneously hypertensive rats
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摘要 目的探讨儿茶酚胺抑素(Catestatin)在高血压发展过程中的变化规律及其对交感神经活性的作用。方法以自发性高血压大鼠(SHR)(SHR组)及其相应的正常大鼠(WKY)(WKY组)作为研究对象。在大鼠不同的发育阶段(6、12和16周龄)检测心率、血压及血浆Catestatin水平。以心率作为交感神经活性的标志。酶联免疫吸附测定Catestatin的浓度,尾套法检测大鼠的血压及心率。结果 SHR组各个周龄的血压、心率均高于相应周龄的WKY组大鼠。SHR组血浆Catestatin浓度高于WKY组(P <0.05),随着周龄的增加,SHR组血浆Catestatin浓度逐渐升高,6周龄为(1.60±0.54)ng/ml,12周龄为(1.76±0.26)ng/ml,16周龄为(3.03±0.57)ng/ml。而WKY组大鼠则保持不变,6周龄为(1.10±0.28)ng/ml,12周龄为(1.05±0.21)ng/ml,16周龄为(1.17±0.12)ng/ml,两组血浆Catestatin的浓度变化趋势有差异(P <0.05)。12周SHR组大鼠尾静脉注射Catestatin,30 min后心率由(417.6±20.9)次/min降到(378.1±15.6)次/min(P <0.05);但血压未下降。结论Catestatin具有抑制心率的作用,可能具有代偿性抑制交感活性的作用。 Objective To investigate the changes of catecholamine catestatin in the development of hypertension and its effect on sympathetic nerve activity.Methods Spontaneously hypertensive rats(SHR)and their littermates Wistar-Kyoto rats(WKY)were used in this study.Heart rate,blood pressure,and plasma catestatin levels were measured at different developmental stages(6,12,and 16 weeks of age)in rats.Heart rate is used as a marker of sympathetic activity.The concentration of catestatin was detected by enzyme-linked immunosorbent assay,and the blood pressure and heart rate of the rats were detected by tail-clamp method.Results The blood pressure and heart rate of SHR in each age group were higher than those of WKY mice of corresponding age.The plasma catestatin concentration in SHR was higher than WKY(P<0.05).The plasma catestatin concentration gradually increased with the increase of week age,(1.60±0.54)ng/ml at 6 weeks,(1.76±0.26)ng/ml at 12 weeks and(3.03±0.57)ng/ml at 16 weeks of age.The WKY rats remained unchanged at(1.10±0.28)ng/ml at 6 weeks of age,(1.05±0.21)ng/ml at 12 weeks of age,and(1.17±0.12)ng/ml at 16 weeks of age.There was a difference in the trend of change(P<0.05).After 12 weeks of SHR,cestastatin was injected into the tail vein.After 30 minutes,the heart rate decreased from(417.6±20.9)times/min to(378.1±15.6)times/min(P<0.05),but the blood pressure did not decrease significantly before injection.Conclusions Catestatin can inhibit the heart rate of SHR,and its gradual increase in SHR plasma may have compensatory inhibition of sympathetic activity.
作者 郭建强 李素娟 贾宇臣 Jian-qiang Guo;Su-juan Li;Yu-chen Jia(Department of Cardiology,The Affiliated Hospital of Inner Mongolia Medical University,Hohhot,Inner Mongolia 010050,China;Department of Gastroenterology,The Affiliated Hospital of Inner Mongolia Medical University,Hohhot,Inner Mongolia 010050,China;Molecular Biology Research Center of Inner Mongolia Medical University,Hohhot,Inner Mongolia 010050,China)
出处 《中国现代医学杂志》 CAS 2019年第5期12-16,共5页 China Journal of Modern Medicine
基金 内蒙古医科大学附属医院一般科研项目(No:NYFYYB)
关键词 高血压 儿茶酚胺抑素 交感神经活性 大鼠 近交SHR hypertension catestatin sympathetic activity rats, inbred shr
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