高效液相色谱-蒸发光散射法测定杏贝止咳颗粒中贝母素甲和贝母素乙含量

Content Determination of Peimine and Peiminine in Xingbei Zhike Granules by HPLC-ELSD

目的建立测定杏贝止咳颗粒中贝母素甲和贝母素乙含量的高效液相色谱-蒸发光散射(HPLC-ELSD)法。方法色谱柱为Phenomenex C18柱(250 mm×4. 6 mm,5μm),流动相为70%乙腈溶液(含0. 08%二乙胺),流速为0. 8 L/min,ELSD漂移管温度为85℃,载气(氮气)流速为2. 1 L/min,雾化器温度为70℃,进样量为10μL。结果贝母素甲和贝母素乙进样量分别在0. 133～2. 655μg和0. 125～2. 547μg范围内与峰面积线性关系良好,精密度、稳定性、重复性试验的RSD均小于2%,加样回收率分别为99. 22%～100. 66%,96. 08%～99. 86%,RSD分别为0. 48%和1. 08%(n=9)。结论该方法操作简便、准确,精密度、稳定性、重复性好,可用于杏贝止咳颗粒中贝母素甲和贝母素乙的含量测定。

Objective To establish an HPLC-ELSD method for the content determination of peimine and peiminine in Xingbei Zhike Granules.Methods The chromatographic column was Phenomenex Ci8 column(250 mmx 4.6 mm,5μm),the mobile phase was 70%acetonitrile solution(containing 0.08%diethylamine),the flow rate was 0.8 L/min,the temperature of ELSD drift tube was 85℃,the flow rate of carrier gas(nitrogen)was 2.1 L/min,the temperature of atomizer was 70℃,and the sample size was 10μL.Results The peimine and peiminine showed good liner relationship in the ranges of 0.133-2.655μg and 0.125-2.547μg,the of preci sion,stability and repeatability were less than 2%.The average recovery rates were were 99.22%-100.66%,96.08%-99.86%,were 0.48%and 1.08%(n=9).Conclusion The method is simple and accurate,it has good precision,stability and repeatability,which can be used for the content determination of peimine and peiminine in Xingbei Zhike Granules.