金花茶黄酮类化合物的生物学功能及提取分离测定方法研究进展

Research Progress in Biological Functions and Methods of Extraction, Seperation, Determination of Flavonoids from Camellia nitidissima

金花茶含有丰富的黄酮类化合物。作为一类重要的天然产物,黄酮类化合物具有多种生物学功能,如抗氧化、降血糖、抗癌、抗肿瘤等,其抗氧化作用最为突出,并构成其他功能的基础。为了实现金花茶中黄酮类化合物的高效提取、分离及测定,开发金花茶黄酮类产品,对黄酮类化合物及金花茶黄酮类成分进行了分析,介绍了黄酮类化合物的多种生物学功能,并就金花茶黄酮类化合物的提取方法(水提法、有机溶剂提取、超声波辅助提取、酶解法)、分离方法(大孔树脂吸附法、色谱法、膜分离法)、测定方法(分光光度法、高效液相色谱法、液相色谱与质谱联用法)进行综述研究。目前金花茶黄酮类化合物的提取方法主要是有机溶剂提取法,其与超声波方法结合效果更佳;金花茶黄酮类化合物的分离大多采用大孔树脂吸附法及色谱法;金花茶黄酮类化合物的测定以分光光度法为主,高效液相色谱法及液相色谱与质谱联用法具有较大应用潜力。

nitidissima is rich in flavonoids. As an important natural product, flavonoids possess a variety of biological functions, such asoxidation resistance, blood sugar lowering, anti-cancer, anti-tumor and other effects. Among these functions, oxidation resistance is most prominent one and forms the basis of other functions. In order to realize the efficient extraction, separation and determination of flavonoids in C. nitidissima and to develop C. nitidissima flavonoids product, this paper analyzed the flavonoids and the flavonoid constituent in C. nitidissima, introduced various biological functions of flavonoids and conducted the summary and research of the extraction (water extraction, organic solvent extraction, ultrasonic assisted extraction and enzymatic hydrolysis), separation (macroporous resin adsorption, chromatography and membrane separation) and determination (spectrophotometry, high performance liquid chromatography,Oliquid chromatography and mass spectrometry) methods of flavonoids in C. nitidissima. At present, the major extraction method of flavonoids in C. nitidissima is the organic solvent extraction method which combined with ultrasonic method, may achieve better extraction effect;the separation of flavonoids in C. nitidissima were mostly conducted by macroporous resin adsorption method and chromatography;spectrophotometry is the major method used for the determination of flavonoids in C. nitidissima while high performance liquid chromatography and liquid chromatography-mass spectrometry have great application potential, which would provide theoretical support for development and utilization of the C. nitidissima.