黄龙病菌感染对长春花SOD和POD活性的影响

Effect of Candidatus Liberibacter asiaticus Infection on the Activities of SOD and POD in Catharanthus roseus

【目的】比较黄龙病菌感染对长春花叶、茎、根3个组织中超氧化物岐化酶(SOD)和过氧化物酶(POD)活性的影响。【方法】采用分光光度计法测定黄龙病菌感染后长春花叶、茎、根3个组织中SOD和POD的活性。【结果】染病长春花中SOD活性随感染时间的增加,逐渐下降,活性最低时,叶、茎和根SOD活性分别只有健康对照的61%、55%和47%。3个组织间SOD活性总体表现为叶>茎>根。染病长春花中POD活性随感染时间的增加呈现先上升后下降的趋势,叶、茎和根POD活性最高出现在嫁接后30、35、35 d,活性分别为健康对照的205%、254%和194%,根中POD活性总体较叶、茎部高。【结论】黄龙病菌感染对长春花各组织中SOD和POD酶的活性均有较大影响,SOD和POD活性的增加对长春花抵抗CaLas侵染起重要作用。

【Objective】The objective is to compare the effects of Candidatus Liberibacter asiaticus(CaLas) infection on the activity of superoxide dismutase (SOD) and peroxidase (POD) in three organs, i.e. leaves, stems and roots of Catharanthus roseus.【Method】The activities of SODand POD in the leaves, stems and roots of C. roseus infected by CaLas were determined by spectrophotometer method.【Result】The activity of SOD in the affected C. roseus decreased gradually with the increase of infection duration. When the activity was the lowest, the activity of SOD in the leaves, stems and roots was only 61%, 55% and 47% respectively of that of the healthy control group. Among these three organs, the leaves had the highest SOD activity while the roots had the lowest. The activity of POD in the affected C. roseus increased first and then decreased with the increase of infection duration. The activity of POD in leaves, stems and roots reached the highest 30, 35 and 35 days after grafting, and was 205%, 254% and 194% respectively of that of the healthy control group. The activity of POD in roots was much higher than that in leaves and stems【Conclusion】The infection of CaLas has a great effect on the activity of SOD and POD in the organs of C. roseus. The increase of SOD and POD activities plays an important role in the resistance of C. roseus to CaLas infection.