曲古抑菌素A对胃癌BGC823细胞增殖及凋亡的影响

Effects of TSA on proliferation and apoptosis in human gastric cancer SGC-823 cells

目的:研究曲古抑菌素A(TSA)对体外培养的胃癌BGC823细胞增殖及凋亡的影响。方法:体外培养人胃癌BGC823细胞,采用CCK8法测定不同浓度(100 nmol/L,200 nmol/L,300 nmol/L,400 nmol/L)及不同时间点(12 h、24 h、48 h)TSA对细胞增殖的影响。用流式细胞术检测48 h不同TSA浓度BGC823细胞的凋亡率。结果:CCK8法检测结果发现TSA对胃癌BGC823细胞有明显的增殖抑制作用,且呈浓度依赖性和时间依赖性。流式细胞术检测结果发现TSA可诱导人胃癌BGC823细胞凋亡,且呈浓度依赖性。结论:TSA可抑制体外培养的胃癌BGC823细胞生长,并可诱导其发生凋亡。

Objective:To study the effect of trichostatin A (TSA) on the proliferation and apoptosis of gastric cancer BGC823 cells in vitro.Methods:The proliferation of human gastric cancer BGC823 cells was detected by CCK8 method at different concentrations (100 nmol/L,200 nmol/L,300 nmol/L,400 nmol/L) and at different time points (12 h,24 h,48 h) Apoptosis rate of BGC823 cells treated with different TSA at 48 h was determined by cell cycle.Results:CCK8 assay showed that TSA had a significant inhibitory effect on gastric cancer BGC823 cells in a concentration-dependent and time-dependent manner.Flow cytometry showed that TSA could induce the apoptosis of human gastric cancer BGC823 cells in a concentration-dependent manner.Conclusion:TSA can inhibit the growth of gastric cancer BGC823 cells in vitro and induce apoptosis.