花生膜联蛋白基因家族成员的结构和表达分析

Structure and expression analysis of the members of peanut annexin gene family

植物膜联蛋白(annexin)是一类钙依赖性磷脂结合蛋白,参与调控植物代谢和生长发育并协同调节抗旱、耐盐等多种抗逆反应,其结构在不同植物中具有物种特异性。为系统分析花生膜蛋白基因家族,对30个花生annexin(annexinof Arachis hypogaea, AnnAh)基因进行了生物信息学分析。结果表明30个AnnAhs不均匀分布在13条染色体上,其中A、B基因组中各有13个和17个。AnnAhs含有2～8个内含子,但大多数含有5～6个内含子。聚类分析表明,植物annexin聚类关系比较复杂,低等植物、单子叶、双子叶植物annexin间隔分布, AnnAhs穿插其中,分布于各个分支中;但在各个小分支中,AnnAhs基本上都与双子叶植物annexin聚在一起,其中与大豆、苜蓿、向日葵亲缘关系较近,其次是拟南芥;但个别AnnAhs与单子叶植物和低等植物annexin聚在一起。30个AnnAhs均无跨膜结构域,其中有16个AnnAhs定位于细胞质,其余定位不明确。对AnnAhs可变剪切分析显示,仅有11个发生可变剪切事件,占38%;根中发生的最多,其次是叶中,种子中最少。根据表达谱数据分析发现, AnnAhs在seed2和根中表达量较高,其次是seed1,在叶中表达量较低。本文可为花生抗性育种提供一定的理论支撑。

Annexin is a kind of calcium-dependent phospholipid binding proteins involved in the regulation of plant metabolism,growth and development,drought resistance and salt tolerance,and its structure is species-specific in different plants.In order to have a systematic analysis of the annexin gene family of peanut,we identified 30 annexin genes from the peanut genome database,and analyzed their characteristics using bioinformatics method.Peanut annexin(annexin of Arachis hypogaea,AnnAh)genes were unevenly distributed on 13 chromosomes,with 13 in A genome and 17 in B genome.There were 2 to 8 introns in the AnnAhs,with 5 to 6 introns in most AnnAhs.Phylogenetic analysis showed that the clustering relationship was complex.The annexins of lower plant,monocotyledonous and dicotyledonous plants were distributed at interval,with AnnAhs inserting in each branch.However,in each small branch,AnnAhs were basically clustered with the dicotyledonous plant annexins,and close to soybean,alfalfa and sunflower,followed by Arabidopsis;but several AnnAhs were associated with monocotyledonous and lower plant annexins.All 30 AnnAhs had no transmembrane domain,and 16 of them were located in cytoplasm,and the others’localization was uncertain.Results of alternative splicing(AS)analysis of AnnAhs showed that only 11 AnnAhs experienced AS,which accounted for about 38% of all AnnAhs;the AS events occurred most in roots,followed by leaves,and the least in seeds.The expression level of AnnAhs was high in seed2 and root,followed by seed1,and lower in leaf.The comprehensive analysis of AnnAhs can provide some theoretical support for peanut resistance breeding.