堆肥添加氧化石墨烯和纳米SiO_2对高羊茅生长的影响

Effects of graphene oxide and nano-SiO_2 on the growth of Festuca arundinacea growing in waste compost

为了探究氧化石墨烯(GO)和纳米SiO_2对高羊茅生长和生理特征的影响,将1.0%GO与不同比例的纳米SiO_2(均为质量分数)与垃圾堆肥基质混合后播种高羊茅,测定高羊茅的株高、生物量、叶绿素含量、丙二醛(MDA)含量以及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)的活性.结果表明:2种钝化剂均能够显著增加高羊茅的株高,对生物量和叶绿素含量没有显著影响,共同添加GO和0.6%纳米SiO_2处理组的高羊茅株高最大.添加2种钝化剂后,高羊茅的MDA含量显著提高,各钝化剂处理组的MDA含量是对照组的2～3倍.单独添加纳米Si O2的处理使SOD、CAT活性提高,POD、APX活性降低.共同添加GO和纳米SiO_2的处理组中,SOD和CAT活性均随着纳米SiO_2添加比例的增加而增加,POD和APX活性则随添加比例的增加而下降.与单独添加纳米SiO_2的处理相比,共同添加GO和纳米SiO_2对酶活性的影响更大.与对照组相比,添加GO和0.6%的纳米SiO_2后,高羊茅的CAT活性提高了65.18%,APX活性降低了51.10%.由此可见,添加GO和纳米SiO_2对高羊茅构成了逆境胁迫,但在生长期内没有显著影响高羊茅的正常生长发育.

In order to explore the effects of graphene oxide(GO)and nano-SiO2 on the growth and physiological characteristics of Festuca arundinacea,F.arundinacea was planted in municipal solid waste(MSW)compost mixed with 1.0%GO and different proportion of nano-SiO(2 mass fraction).The plant height,biomass,chlorophyll content,malondialdehyde(MDA)content,super-oxide dismutase(SOD),peroxidase(POD),catalase(CAT)and ascorbic acid peroxidase(APX)activity were measured.The results showed that two kinds of passivators significantly increased the plant height of F.arundinacea and had no significant effect on biomass and the chlorophyll content.The addition of GO and 0.6%nano-SiO2 together resulted in the highest plant height.GO and nano-SiO2 significantly increased MDA content.MDA contents in four GO and nano-SiO2 treatments were 2-3 times as those of the control.The addition of nano-SiO2 alone increased SOD and CAT activities,and decreased POD and APX activities.When GO was applied together with nano-SiO2,the SOD and CAT activities increased with the increase of the proportion of nano-SiO2,and the activities of POD and APX decreased with the increase of the proportion of nano-SiO2.Compared with the addition of nano-SiO2 alone,combined addition of GO and nano-SiO2 had a greater effect on the enzyme activity.Compared with the control,the CAT activity increased 65.18%and the APX activity decreased 51.10%in the treatment of GO and 0.6%nano-SiO2.It can be concluded that the addition of GO and nano-SiO2 caused stress to F.arundinacea,but did not significantly affect the normal growth and development of F.arundi-nacea during the growth period.