平欧榛子甾醇和VE指纹图谱构建与分析

Establishment and Analysis of Flat-European Hybrid Hazelnut Sterol and VE Fingerprints

为了榛子资源品种改良、榛子油品质评价及质量控制,采用GC-MS技术和HPLC技术结合系统聚类、相似度分析法,构建不同平欧榛子活性物质甾醇和VE的标准指纹图谱,并就各样品甾醇及VE的指纹图谱进行分析。结果表明,14个样品甾醇和VE的含量有一定差异;根据相似度分析,分别建立了平欧榛子甾醇和VE的标准指纹图谱,且样品间甾醇色谱图相似度均在0.98以上,整体相似性较高,而样品间VE色谱图相似度存在较大差异,比较发现同一地区不同品种间VE相似度高于同一品种不同地区样品间相似度;聚类分析显示,平欧榛子甾醇根据品种划分为4个类别,而VE则根据种植区域也划分为4个类别,品种和种植区域分别是影响平欧榛子甾醇和VE组成的重要因素。

For the hazelnut varieties breeding,hazelnut oil quality evaluation and control,the Flat-European hybrid hazelnut sterol standard fingerprints were established by GC-MS technology,and the Flat-European hybrid hazelnut VE standard fingerprints were established by HPLC technology,meanwhile,the similar degree were analyzed combination with system clustering and similarity analysis. The results indicated that the sterol and the VE contents of 14 kinds of samples have certain differences;The similarities showed that the sterol and VE in samples were all similar;In addition,sterol and VE in the 14 kinds of Flat-European hybrid hazelnut samples could be respectively according to cultivar and region clustering 4 categories,that varieties and planting area were important factors for affecting sterol and VE Flat-European hybrid hazelnut.