两种多糖对齐口裂腹鱼免疫性能的影响

Effect of Two Kinds of Polysaccharide on Immune Performance of Schizothorax Prenanti

探讨OKGM、OKGMS两种多糖对齐口裂腹鱼免疫性能的影响,将齐口裂腹鱼随机分为7组,两种多糖添加剂量分别为质量分数0.4%、0.8%、1.6%,饲喂60 d,测定齐口裂腹鱼血清免疫及抗氧化指标,并采用RT-PCR技术检测组织MyD88、IRF7基因相对表达量。结果表明,OKGMS_(1.6)组能显著提高齐口裂腹鱼血清IgM活性、ACH50含量;OKGMS_(0.4)、OKGMS_(0.8)、OKGM_(0.4)、OKGM_(0.8)组均能显著降低血清MDA的含量;OKGMS与OKGM均能提高血清SOD活性;OKGMS能上调齐口裂腹鱼肠道MyD88基因mRNA相对表达量及肝脏、脾脏、中肾IRF7基因mRNA相对表达量;OKGM能上调肠道、头肾、中肾MyD88基因mRNA相对表达量及肠道、肝脏、脾脏、中肾IRF7基因m RNA相对表达量。结果表明:适量添加OKGM、OKGMS能提高齐口裂腹鱼抗氧化活性及免疫性能。

The experiment was conducted to investigate the effects of oxidized konjac glucomannan and oxidized konjac glucomannan sulfates on immune performance of Schizothorax prenanti. The Schizothorax prenanti were randomly divided into 7 groups and each group included 3 replicates of 20 fish. The experiment lasted for 60 days with the treatment groups were contained 0.4%、0.8%、1.6% of OKGM and OKGMS. And body composition and serum biochemical indices were measured and MyD88、IRF7 mRNA level in tissue were determined by RT-PCR. Results:Content of IgM、ACH50 of OKGMS1.6 were significantly more than the control group;The content of MDA was significantly lower of group OKGMS0.4、OKGMS0.8、OKGM0.4、OKGM0.8 than the control group;OKGMS、OKGM both increased the activity of SOD;OKGMS could up-regulate MyD88 mRNA level in gut and IRF7 mRNA level in liver、spleen and mesonephros;OKGM could increase MyD88 mRNA level in gut、headkidney and mesonephros and IRF7 mRNA level in gut、liver、spleen and mesonephros. Conclusions:Add the appropriate amount of OKGM and OKGMS could increase the antioxidant activity and immune functions of Schizothorax prenanti.