一种氧杂蒽染料的合成及在水中的光谱性能研究

Synthesis of A New Xanthene Dye and Its Optical Properties in Water under Different pH

通过酸催化的缩合反应合成了一种荧烷染料2,研究了其在不同溶剂中的光谱行为。对化合物2的游离氨基进行酰化,得到了新型的荧光染料3。利用核磁共振波谱、高分辨质谱对3进行了结构表征。考察了化合物3在不同的p H水溶液的紫外吸收和荧光光谱变化。研究表明,该化合物在生理p H范围内保持螺环内酯开启状态,并保持较稳定的荧光强度,有作为生物标记及生物成像材料的潜力。

A Fluoran dye 2 was synthesized by the acid-promoted condensation reaction and its optical behaviors in various solvents were investigated. Subsequently, the free amino-group of 2 was further modified via acylation reaction to offer a new fluorescent dye 3 . The structure of 3 was characterized by nuclear magnetic resonance spectrum and high resolution mass spectrometry. UV-vis and fluorescent spectra of 3 in water under different pH were examined. The results indicated that the stable fluorescence in the physiological pH range was ascribed to the spirolactone ring opening of compound 3 , which made 3 a promising material for biomarker and bioimaging.