JMJD1A促进胶质瘤增殖侵袭的分子机制探究

The Role of JMJD1A in Regulating Glioma Proliferation and Invasion

目的探讨JMJD1A促进胶质瘤增殖侵袭的分子机制。方法通过Western Blot方法和利用TCGA数据库,分析胶质瘤组织中JMJD1A的表达情况。在胶质瘤细胞系U87和U251中,通过siRNA瞬时干扰JMJD1A的表达;利用体外实验方法检测JMJD1A干扰对细胞功能变化的影响。结果与正常脑组织相比,胶质瘤组织中JMJD1A蛋白及mRNA水平均显著升高;干扰JMJD1A表达后,胶质瘤细胞的增殖及侵袭能力显著下降。结论 JMJD1A具有促进胶质瘤细胞增殖和侵袭的作用。

Objective To investigate the role of JMJD1A in regulating glioma proliferation and invasion.Methods Western Blot and TCGA dataset were used to determine JMJD1A level in glioma specimens;siRNAof JMJD1A was used to interfere JMJD1A expression in glioma cell lines U87 and U251;In vitro experiments were used to determine cell proliferation and invasion.Results Compared to normal brain tissues,both JMJD1A mRNA and protein level were upregulated in glioma tissues;JMJD1A interfering could significantly inhibit glioma cell proliferation and invasion.Conclusion JMJD1A exhibited critical roles in glioma development by regulating cell proliferation and invasion.