复合生物保鲜剂结合冰温贮藏对红鳍东方鲀的保鲜效果

Preservation effect of complex biological preservative combined with ice temperature storage on Takifugu rubripes

为了延长红鳍东方鲀鱼块的货架期,利用响应面Box-Behnken原理设计柠檬酸、百里酚和海藻酸钠3因素3水平响应面分析实验,建立以菌落总数为响应值的二次多项式回归模型。并通过pH、汁液流失率、挥发性盐基氮(TVB-N)、硫代巴比妥酸(TBA)、菌落总数及优势腐败菌假单胞菌等指标测定,对最佳配比的复合生物保鲜剂的保鲜效果进行了验证。结果显示,冰温下,柠檬酸、百里酚、海藻酸钠的最优配比分别为0.68%、1.45%、1.94%,贮藏30 d时菌落总数为最低值5.50 lg (CFU/g),并且3种保鲜剂两两间均存在极显著的交互作用。经验证,该复合生物保鲜剂可以显著抑制微生物的增长,有效降低贮藏过程中的TVB-N含量、TBA含量,显著减缓了鱼块的腐败变质发生,货架期较对照组的15 d延长至30 d。

The aim of this paper was to optimize complex biological preservatives consisting of citric acid,thymol and sodium alginate using three-variable,three-level Box-Behnken design to prolong the shelf life of Takifugu rubripes.A quadratic polynomial regression model was established with total plate counts as the response value.The preservation effects of complex biological preservatives were verified by measuring pH,drip loss,TVB-N,TBA,total plate counts and Pseudomonas sp..The result showed that the optimal ratio of the complex biological preservatives at ice temperature is as follows:0.68%citric acid,1.45%thymol and 1.94%sodium alginate.In that condition,the lowest total plate count was 5.50 lg(CFU/g)on the 30th day.What’s more,citric acid and thymol,citric acid and sodium alginate,as well as thymol and sodium alginate had a significant interactive effect.The optimal complex biological preservatives could significantly inhibit the growth of microorganisms,and reduce the contents of TVB-N and TBA.It was proved that the complex biological preservatives could effectively delay the deterioration of T.rubripes,prolonging the shelf life of 15 d to 30 d.