载阿霉素碳量子点纳米粒的制备及其对人肺癌细胞A549的体外抗肿瘤作用研究

Preparation of Doxorubicin-loaded Carbon Quantum Dot Nanoparticles and Its Anti-tumor Effect on Human Lung Cancer Cell A549 in Vitro

目的制备载阿霉素碳量子点纳米粒并考察其体外抗肿瘤作用。方法采用水热合成法一步合成羧基化碳量子点(CDs-COOH),阿霉素(DOX)的氨基通过酰胺反应与CDs-COOH的羧基共价连接,合成CDs-DOX载药碳量子点纳米粒。利用荧光分光光度计、红外光谱仪、紫外分光光度计、透射电镜对CDs-COOH进行了研究;利用红外光谱仪、紫外分光光度计、粒度仪对CDs-DOX进行了表征;采用MTT法检测CDs-DOX对人肺癌细胞A549的毒性。结果通过透射电镜观察CDs-COOH呈球形,分布较均匀,平均粒径5 nm左右,荧光分光光度计结果进一步验证其尺寸均匀这一性质,通过紫外分光光度计、红外光谱仪等确证CDs-COOH和CDs-DOX制备成功。MTT实验表明,CDs-DOX对人肺癌细胞A549具有较强的细胞毒性。结论载阿霉素碳量子点纳米粒CDs-DOX具有一定的体外抗肿瘤作用,并具有开发成抗肿瘤递药系统的重要潜力。

Objective To prepare drug-loaded carbon quantum dots nanoparticles and investigate their anti-tumor effects in vitro. Methods The carbon-carbon quantum dots (CDs-COOH) were synthesized in one step by hydrothermal method. The amino group of doxorubicin (DOX) was covalently linked to the carboxyl group of CDs-COOH through an amide reaction to synthesize CDs-DOX loaded carbon quantum dot nanoparticles. CDs-COOH was studied by fluorescence spectrophotometer, infrared spectrometer, ultraviolet spectrophotometer and transmission electron microscopy. CDs-DOX was characterized by infrared spectroscopy, ultraviolet spectrophotometer and particle size analyzer. The toxicity of CDs-DOX to human lung cancer cell A549 was tested by MTT assay. Results The transmission electron microscopy showed that the CDs-COOH was spherical and the average particle size was about 5 nm. The results of fluorescence spectrophotometer further verified the uniform size of CDs-COOH. The UV-visible spectrophotometer and infrared spectroscopy results also confirmed the successful preparation of CDs-COOH and CDs-DOX. MTT experiments showed that CDs-DOX had high cytotoxicity to A549 when compared with free DOX. Conclusion The drug-loaded carbon quantum dots nanoparticles CDs-DOX were successfully prepared. It had anti-tumor effects in vitro and showed great potential in the development of anti-tumor drug delivery.