Fenton氧化对微生物群落结构和功能基因的影响

Effects of Fenton Oxidized Crude Oil Contaminated Soil on Indigenous Microbial Community Structure and Functional Genes

通过使用H_2O_2和Na_2CO_3·1.5H_2O_2所形成的两种类Fenton试剂对原油污染的土壤进行氧化修复,在比较两种试剂氧化效果的基础上,采用16s rDNA技术对氧化后土壤中土著微生物的群落结构和功能基因的影响进行了比较。结果表明:Na_2CO_3·1.5H_2O_2所形成的类Fenton试剂对原油的降解效果较好,原油降解率达到50.48%;两种试剂氧化后土壤中的优势菌种均发生变化,由原来的放线菌门(Actinobacteria)变为变形菌门(Proteobacteria),同时氧化后土壤中的总功能基因含量下降,由原来的58 093 193个分别下降到36 282 211个和32 355 524个,而各种通路基因在总基因的占比变化不大。

In this paper, two kinds of Fenton reagents formed by H2O2 andNa2CO3·1.5H2O2 were used to oxidize and remediate crude oil-contaminated soils. Based on the comparison of the oxidation effects of the two reagents, 16s rDNA technology was applied to evaluate the community structure and functional genes of indigenous microorganisms in oxidized soils. The results showed that, the Fenton-like reagent formed by Na Na2CO3·1.5H2O2 had a better degradation effect on crude oil, with a degradation rate of reached 50.48%. The dominant bacteria in soil changed from Actinobacteria to Proteobacteria after oxidation of the two reagents. The content of total functional genes decreased from 58093193 to 3628211 and 3235524, respectively, while the proportion of various pathway genes in total genes remained relatively stable.