四氢姜黄素对H9c2细胞缺血再灌注损伤的影响及机制研究

Protective effects of tetrahydrocurcum on myocardial ischemia reperfusion injury of H9c2 cells

目的探究四氢姜黄素(THC)预处理能否减轻H9c2细胞缺血再灌注损伤(IRI)并探究其相关机制。方法H9c2细胞置于缺血液中处理1 h,再更换至正常DMEM细胞培养基中4 h模拟在体心肌IRI。缺血液处理前24 h加入THC。将培养细胞分为以下4组:对照(Control)组; THC预处理(THC)组;模拟缺血再灌注(SIR)组; THC+SIR(THC+SIR)组。再灌注4 h后检测各组H9c2细胞活力、活性氧(ROS)产量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)与谷胱甘肽过氧化物酶(GSH-Px)活性、凋亡率、Bax、Bcl-2和Caspase-3蛋白表达情况。结果与SIR组相比,THC可明显增加细胞活力、Bcl-2表达、SOD与GSH-Px活力;降低凋亡率、Bax与Caspase-3表达、ROS产量和MDA含量(均P <0.05)。结论 THC预处理可通过降低氧化应激和凋亡,最终减轻H9c2细胞IRI。

Objective To investigate the protective effects and underlying mechanisms of tetrahydrocurcumin(THC)pretreat.ment on ischemia reperfusion injury(IRI)of H9c2 cells.Methods To mimic the in vivo ischemia reperfusion model,H9c2 cells were exposed to an ischemic buffer for 1 h followed by serum-free DMEM for 4 h.Prior to this procedure,cells were treated with or without THC.They were divided into four groups:Control group,THC group,SIR group and SIR+THC group,respectively.Four hours after the reperfusion,cell viability,reactive oxygen species(ROS)production,malonaldehyde(MDA)content,activities of superox.ide dismutase(SOD)and glutathione peroxidase(GSH-Px)as well as the expression levels of Bax,Bcl-2 and Caspase-3 were measured in each group.Results Compared with SIR group,THC pretreatment increased the cell viability,the expression of Bcl-2 and the activities of SOD and GSH-Px in H9c2 cells.In addition,THC also significantly decreased the apoptotic ratio,the expression of Bax and Caspase-3,ROS production and MDA content.Conclusion THC pretreatment could effectively attenuate myocardial IRI by reducing SIR-induced oxidative stress and apoptosis.