瘦素基因启动子区G-2548A多态性与2型糖尿病肾病的关系

The correlation between polymorphism G-2548A in the promoter of leptin gene and the nephropathy in type 2 diabetes mellitus

目的探讨瘦素基因启动子区G-2548A(rs7799039)多态性与2型糖尿病肾病的关系。方法选取2016年5月—2017年5月在华北理工大学附属医院内分泌病区住院的2型糖尿病患者416例。其中,无糖尿病肾病组224例,男性102例,女性122例,平均年龄(56.2±11.1)岁;2型糖尿病肾病组192例,男性96例,女性96例,平均年龄(58.3±11.5)岁。采用聚合酶链反应及限制性片段长度多态性方法测定G-2548A基因型,比较两组基因型和等位基因多态性的不同。结果①2型糖尿病肾病组患者血清瘦素水平、尿素氮、肌酐、糖化血红蛋白、收缩压、舒张压高于无糖尿病肾病组(P<0.05);两组尿酸和血脂相关指标比较,差异无统计学意义(P>0.05)。②两组患者G-2548A基因型和等位基因分布频率比较,差异有统计学意义(P<0.05)。结论瘦素基因启动子区G-2548A基因型分布、等位基因多态性与2型糖尿病肾病有关,其中A等位基因可能是2型糖尿病肾病发生、发展的易感基因。

To investigate the relationship between leptin gene promoter G-2548A (rs7799039) polymorphism and type 2 diabetic nephropathy. Methods A total of 416 Patients with type 2 diabetes were selected, who were in hospital in the affiliated hospital of North China University of Science and Technology from May 2016 to May 2017. There were 224 cases in no diabetic nephropathy group with 102 males and 122 females, and the average age was (56.2±11.1). The other 192 cases were in type 2 diabetic nephropathy group with 96 females and 96 females, and the average age was (58.3±11.5). The study adopted the method of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) to determine the genotypes of G-2548A and comparison of differences between genotypes and allelic polymorphisms. Results ① There were statistical significance of the differences in serum leptin levels, urea nitrogen, creatinine, glycosylated hemoglobin, systolic blood pressure and diastolic blood pressure among no diabetic nephropathy group and type 2 diabetic nephropathy group (P < 0.05), while there was no statistical significance of the differences in related index of uric acid and blood lipids (P > 0.05).② The genotype distribution and allele frequency of G-2548A in diabetic nephropathy group and type 2 diabetic nephropathy group were statistically significant (P < 0.05). Conclusions The distribution of G-2548A genotype in leptin gene promoter region and allele polymorphism have some correlation with type 2 diabetic nephropathy. The A allele may be a susceptible gene for the development of type 2 diabetic nephropathy.