长链非编码RNA THOR促进宫颈癌细胞C33A增殖

The function of long non-coding RNA THOR in cervical cancer cell C33A

目的研究长链非编码RNATHOR对宫颈癌C33A细胞生长的调控作用。方法 qRTPCR分别检测15对肿瘤组织/癌旁组织和宫颈癌细胞系C33A、siHa、正常宫颈鳞状上皮细胞H8中的LncRNA THOR表达水平。在宫颈癌细胞系C33A中,siRNA沉默LncRNA THOR后利用CCK8和平板克隆形成实验检测细胞增殖,流式细胞学实验检测细胞周期,使用western blot检测细胞周期蛋白变化。结果 qRT-PCR结果显示15例组织中12例肿瘤组织LncRNA THOR表达明显高于癌旁组织,宫颈癌细胞系C33A、siHa中LncRNA THOR表达也明显高于宫颈鳞状上皮细胞H8细胞。在宫颈癌细胞系C33A中沉默LncRNA THOR后,CCK8结果显示细胞增殖受到抑制,平板克隆形成实验表现为细胞克隆明显减少。流式细胞学周期分析发现G0/G1期细胞比值增高。western blot实验显示周期蛋白cyclinD 1和cyclinE1在蛋白水平下降,p27和p21蛋白水平增高。结论 LncRNATHOR在宫颈癌中高表达,沉默LncRNA THOR可以抑制宫颈肿瘤细胞增殖。

Objective To reveal the relationship between long non-coding RNA THOR and cervical cancer cell. Methods We confirmed the expression of LncRNA THOR in 15 pairs of cervical cancer tissues and adjacent normal tissues with qRT-PCR. And then we investigated the expression of LncRNA THOR in cervical cancer cell lines C33A,siHa and cervical squamous epithelium cell line H8 by using the same technique. After silencing lncRNA THOR in C33A cells,we conducted CCK-8 assay and colony formation assay to see the changes of proliferation. With flow cytometry,we tested cell cycle to support the previous results. Also,the changes of cyclin were assayed by western blot. Results According to the results of qRT-PCR,the expression of LncRNA THOR was significantly higher in 14 out of 15 cervical cancer tissues than the adjacent normal tissues,and the same phenomenon showed in cervical cancer cell lines C33A and siHa comparing with cervical squamous cell H8. Silencing LncRNA THOR in C33A cells,cell proliferation was suppressed as the CCK-8 assay showed,which corresponded with the less formation of cell colony in colony formation assay. Flow cytometry also showed the decrease of the G0/G1 cells. Following the silencing of LncRNA THOR,the cyclin D1 and cyclin E1 were down-regulated while the p27 was up-regulated according to the western blot. Conclusion LncRNA THOR exhibited high expression level in cervical cancer cells,and silencing LncRNA THOR suppressed the proliferation of it.