外泌体lncRNA CCAT1在胰腺癌中的表达及其临床意义

Expression and clinical significance of exosomal long non-coding RNA CCATl in pancreatic cancer

目的探讨长链非编码RNA(lncRNA)CCAT1在胰腺癌血清外泌体中的表达及临床意义。方法术前收集66例胰腺癌患者及66例正常健康者的血清,采用差速离心法收集患者外周血中的外泌体。以病变组织病理诊断结果为金标准,统计外泌体中lncRNA CCAT 1诊断胰腺癌的敏感性,特异性和诊断准确率。收集患者的临床病理特征,分析IncRNA CCAT1与临床相关因素的关系。结果外泌体中lncRNA CCAT1检测胰腺癌的敏感度、特异性和准确性分别为89.4%、87.9%和89.4%,显著高于CA19-9(P<0.05); Kaplan-Meier分析提示外泌体lncRNA CCAT1表达与胰腺癌患者总体预后有关;Cox回归分析提示外泌体lncRNA CCAT1是影响胰腺癌患者不良预后的独立因素(P<0.05)。结论 LncRNA CCAT1在胰腺癌外泌体中显著高表达,对于早期诊断胰腺癌具有很高的敏感度、特异性和准确性,可作为预测胰腺癌患者不良预后的独立因素。

Objective To investigate the expression level and clinical significance of exosomal long non-coding RNA CCAT1 in pancreatic cancer. Methods The serums of 66 patients with pancreatic cancer before operation and 66 normal healthy people as control were collected and the exosomes were isolated by ultracentrifugation. The detection sensitivity and specificity of exosomal lncRNA CCAT1 were calculated by using the pathological diagnosis. The clinicopathological features were collected to analyze the clinical relationship between the exosomal lncRNA CCAT1 expression and clinicopathological features. Results The detection sensitivity,specificity and accuracy of lncRNA CCAT1 in pancreatic cancer were 89.4%,87.9% and 89.4%,respectively. Kaplan-Meier analysis showed that the expression levels of lncRNA CCAT1 was related to overall survival time of the patients of pancreatic cancer. Cox regression analysis showed that lncRNA CCAT1 expression was an independent risk factor of pancreatic cancer patients. Conclusion lncRNA CCAT1 was over-expressed in pancreatic cancer exosomes,and exosomal lncRNA CCAT1 deserved as an independent prognosis factor of pancreatic cancer.