同轴静电纺RSF载VEGF纳米纤维膜的制备与研究

Preparation and study of coaxial electrospinning RSF nanofiber membranes loaded with VEGF

为进一步提高再生丝素蛋白(RSF)在生物组织工程中的应用潜力,通过同轴静电纺技术,以RSF溶液为壳层、血管内皮生长因子(VEGF)和胎牛血清为芯层,制备纳米纤维膜。然后通过扫描电子显微镜、透射电子显微镜、ELISA试剂盒等手段,对不同芯层流速下制备的纳米纤维膜的纤维形态和壳芯结构及VEGF释放性能进行对比分析。结果表明:在固定其他因素的情况下,随着芯层流速减小,纤维形态由扁平带状向圆柱形转变,芯层厚度减小,纤维直径下降,纤维的壳芯结构趋于稳定;当壳层的RSF溶液浓度为14 g/m L时,在壳层流速为0. 80 m L/h、芯层流速为0. 05 m L/h的条件下,纤维壳芯结构稳定,VEGF释放性能较优。

In order to further improve the application potential of regenerated silk fibroin(RSF)in biological tissue engineering,nanofiber membranes with RSF solution as shell layer,and vascular endothelial growth factor(VEGF)and fetal bovine serum as core layer were prepared by coaxial electrospinning technology.Then,the morphology and shell-core structure of fiber and the VEGF release properties of the nanofiber membranes prepared at different core flow velocities were compared by scanning electron microscopy,transmission electron microscopy,ELISA kits,etc.The results showed that,in the case other factors fixed,as the core flow velocity decreased,the morphology of fiber changed from flat ribbon to cylindrical shape,the thickness of the core layer reduced,the fiber diameter decreased,and the core structure of fiber tended to be stable.When the concentration of RSF solution on the shell layer was 14 g/mL,under the conditons of the shell flow velocity at 0.80 mL/h and the core layer flow velocity at 0.05 mL/h,the shell-core structure of fiber was stable,and the release performance of VEGF was superior.