p44/WDR77基因与细胞周期蛋白D3在非小细胞肺癌中的表达及相关性研究

Research on the expression and correlation of p44/WDR77 and Cyclin D3 in NSCLC

目的:检测雄激素受体辅助因子(p44/WDR77)基因与细胞周期蛋白D3(CyclinD3)在非小细胞肺癌(NSCLC)中的表达,分析二者在促进NSCLC发生发展过程中的作用。方法:收集190例原发性NSCLC患者的肿瘤组织配对标本,采用免疫组织化学法检测p44/WDR77、CyclinD3在NSCLC组织与相应正常组织中,以及在不同NSCLC患者不同年龄、性别及有无淋巴转移组中的表达情况,统计学分析二者间的相关性及其在临床病理因素中的差异性。结果:p44/WDR77和Cyclin D3定位于细胞核与细胞质中,在NSCLC组织中高表达,癌组织与正常组织中阳性表达率比较差异有统计学意义(Z=-11.379,Z=-11.386;P<0.05)。在不同年龄患者中p44/WDR77的表达差异有统计学意义(Z=-2.641,P<0.05);p44/WDR77和CyclinD3在腺癌、鳞癌间表达均差异无统计学意义(Z=-1.777,Z=-1.252;P>0.05);不同病理分化程度、不同TNM分期的NSCLC组织中p44/WDR77与CyclinD3的阳性表达率比较差异均有统计学意义(x^2=17.077,x^2=11.842,x^2=6.233、x^2=10.273;P<0.05)。CyclinD3的表达差异与患者淋巴结转移具有统计学意义(Z=-11.386,P=0.036);在NSCLC和鳞癌中,p44/WDR77和CyclinD3表达呈正相关(r=0.231,r=0.283;P<0.05)。结论:p44/WDR77和Cyclin D3是NSCLC组织与相应正常组织表达的差异蛋白,二者的过表达可能在NSCLC的发生发展过程中起作用。

Objective: To detect the expression of p44/WDR77 and Cyclin D3 in non-small cell lung cancer(NSCLC), and analyze the effect of them in promoting the occurrence and development process of NSCLC. Methods: Matched specimens of tumor tissue of 190 patients with primary NSCLC were collected. The expressions of p44/WDR77 and Cyclin D3 in NSCLC tissues and corresponding normal tissues, as well as in different age, different sex, lymphatic metastasis group and non-lymphatic metastasis group of different patients with NSCLC were detected by immunohistochemistry. The correlation of them and the variation of them in clinicopathological factors were further analyzed as statistical method. Results: Positive staining of p44/WDR77 and Cyclin D3 were in cytoplasm and nucleus. And the expressions of them were high in NSCLC. The positive expression rates of the p44/WDR77 and Cyclin D3 protein in NSCLC was significantly higher than those in the corresponding normal tissue(Z=-11.379, Z=-11.386, P<0.05). The difference of expressions of p44/WDR77 among different age groups was significant(Z=-2.641, P<0.05). There were no significant differences in the expressions of p44/WDR77 and Cyclin D3 between adenocarcinoma and squamous cell carcinoma(Z=-1.777, Z=-1.252, P >0.05). There were significant differences in the positive expressions of p44/WDR77 and CyclinD3 of NSCLC tissues among different pathological grades and among different TNM stage(x^2=17.077, x^2=11.842, x^2=6.233, x^2=10.273, P<0.05), respectively. The difference of Cyclin D3 expression was significantly correlated with lymph node metastasis(Z=-11.386, P=0.036). The expression level of p44/WDR77 was positively related with that of Cyclin D3 in NSCLC and squamous cell carcinoma(r=0.231, r=0.283, P<0.05), respectively. Conclusion: Our data suggest that p44/WDR77 and Cyclin D3 were differentially expressed proteins in NSCLC tissues and corresponding normal tissues, and the over-expressed proteins being crucial in the process of occurrence and development of NSCLC.