美洲大蠊提取物CⅡ-3 UPLC指纹图谱研究

UPLC Fingerprints of Periplaneta americana Extract CⅡ-3

目的:建立美洲大蠊提取物CⅡ-3超高效液相色谱(Ultra Performance Liquid Chromatography,UPLC)指纹图谱,评价不同批次CⅡ-3样品的质量。方法:采用Phenomenex Gemini 5u C18110A(250 mm×4.6 mm)色谱柱分析美洲大蠊提取物CⅡ-3;柱温28℃;检测波长214 nm;流动相为乙腈-水(0.1%甲酸),进行梯度洗脱。依据相似度评价结果和化学计量学方法进行不同批次CⅡ-3质量评价。结果:建立了12批CⅡ-3指纹图谱,标定共有峰21个,其中11批样品相似度大于0.900。结论:首次建立了美洲大蠊提取物CⅡ-3的UPLC指纹图谱,为CⅡ-3质量控制提供科学依据。

Objective: To establish UPLC fingerprints of Periplaneta americana to assess the quality of different batchs of CⅡ-3 samples. Methods: The analysis of Periplaneta americana extract CⅡ-3 was performed in Phenomenex Gemini 5u C18 110A column (250 mm × 4.6 mm)and the column temperature was 28 ℃. And acetonitrile-water(0.1% formic acid)was the mobile phase of gradient elution, set at 214 nm wavelength. The quality of C Ⅱ- 3 was assessed according to the similarity evaluation and the chemometrics method. Results: The UPLC fingerprints of 12 batches of CⅡ-3 were established. There were 21 common peaks and fingerprints similarity of 11 batches samples was greater than 0.900. Conclusion: It is the first time that the UPLC fingerprint of CⅡ-3 has been established, which provides a scientific basis for the quality control of Periplaneta americana extract CⅡ-3.