用于水产品中甲醛、双氧水和二氧化硫同时快速检测的微流控芯片系统研制

Development of microfluidic chip system for simultaneously rapid detection of formaldehyde,hydrogen peroxide and sulfur dioxide in aquatic products

基于微流控技术与分光光度法,研制了一套用于水产品中甲醛、双氧水和SO_2快速检测的微流控芯片系统。该系统在一次性扇形微流控芯片上集成了进样、显色反应及检测单元,每张芯片可同时检测甲醛、双氧水和SO_2三种指标。结果显示,微流控芯片系统能够在5 min内实现对水产品中甲醛、双氧水、SO_2三种指标的准确检测,检出限分别可达到0.3、0.4、0.2 mg/L,回收率在92.38%～107.98%,相对标准偏差均低于4%。微流控芯片系统能够实现对水产品中甲醛、双氧水和SO_2进行现场、快速、全自动、高通量检测,适合基层非专业人员开展筛选需求,体现了微流控芯片在食品快速检测应用中的巨大潜力。

Based on microfluidic technology and spectrophotometry,a microfluidic chip system for rapid detection of formaldehyde,hydrogen peroxide,and sulfur dioxide in aquatic products was developed. This disposable fan-shaped microfluidic chip was integrated with sampling,color reaction,and detection chamber. Each chip could detect 3 indexes such as formaldehyde,hydrogen peroxide,and sulfur dioxide at the same time. The results showed that the microfluidic chip system could accurately detect formaldehyde,hydrogen peroxide,and sulfur dioxide in aquatic products in 5 minutes with detection limits of 0.3 mg/L,0.4 mg/L,and 0.2 mg/L,respectively. The recovery rate was 92.38%-107.98%,and the relative standard deviation was less than 4%. The microfluidic chip system can realize on-site,rapid,automatic,and high throughput detection of formaldehyde,hydrogen peroxide and sulfur dioxide in aquatic products,which is suitable for unprofessional individuals to screen the products,showing a great potential of applying microfluidic chip in rapid detection for foods.