茯苓菌种液体发酵条件及栽培基质优化分析

Optimization of Poria cocos liquid fermentation conditions and cultivation substrate

本论文对茯苓液体菌种培养条件和栽培基质进行优化。利用摇床在25℃恒温条件下培养,通过单因素和响应面优化实验对茯苓液体发酵条件进行分析,从茯苓液体发酵中摇瓶转速、接种量、发酵时间等因素对茯苓液体发酵的最优条件进行了探讨,结果表明:以150 m L液体培养基进行培养,摇瓶转速为177 r/min,接种量为6.62 m L,发酵时间为8 d,茯苓液体发酵的条件最佳;茯苓最佳的栽培基质配方为:32%草灰、26%玉米芯、18%米糠、16%麸皮、6%豆粕、1%石膏、1%石灰,生物学效率为64%。

In this paper, the conditions of culturing Poria cocos liquid strain and culturing matrix were optimized using the table in 25 ℃ conditions. The fermentation conditions of Poria cocos were analyzed by single factor and response surface optimization experiment.According to the experimental results, the optimal conditions were obtained as follows: culture on 150 mL liquid medium, rotation speed of shaking flask was 177 r/min, and inoculation amount was 6.62 mL, and fermentation time was 8 days. Highest biological efficiency value was 64%. Using a cultivation substrate consisting of 32% straw ash, 26% corn cobs,18% rice hulls, 6% soybean powder, 16% wheat bran and 1% gypsum and 1% lime.