摘要
为了寻找新型天然抗氧化剂,对香水莲花(Nymphaea hybrid)多糖粗提物进行了除蛋白纯化并测定了纯化产物的抗氧化活性。实验结果表明,三氯乙酸法(TCA)的除蛋白效果优于Sevage法和TCA-Sevage法,其蛋白清除率为70.49%,多糖损失率29.24%。TCA法除蛋白后的多糖,对1,1-二苯基-2-三硝基苯肼(DPPH)自由基和羟自由基都有较强的清除能力,且与浓度呈量效关系,有一定的还原能力,对脂质过氧化抑制效果明显,具有一定的抗氧化活性。
The present study compared the deproteinzation methods for crude polysaccharide from Nymphaea hybrid and investigated antioxidant activity of the purified products.The TCA methods gave the best results(with 70.49%protein removal and 29.24%polysaccharide loss)followed by Sevage and Sevage-TCA methods.The product purified by TCA method provided remarkable scavenging effects against DPPH radical and hydroxyl radical(·OH)with a dose dependent manner.Besides,inhibitory effect of the product on lipid peroxidation was observed,showing its antioxidant capacity.
作者
丛恒雪
胡国胜
王文翠
谢先辉
姚雷
卢静
CONG Heng-xue;HU Guo-sheng;WANG Wen-cui;XIE Xian-hui;YAO Lei;LU Jing(School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China)
出处
《上海交通大学学报（农业科学版）》
2018年第6期45-49,共5页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
瑞生生物-上海交大芳香植物产业科技示范工程(15H10000536)
关键词
香水莲花
多糖
蛋白质
抗氧化活性
Nymphaea hybrid
polysaccharide
protein
antioxidant activity
