食品中大肠埃希氏菌O26血清型检测方法的建立

Establishment of PCR method for detection of Escherichia coli O26

大肠埃希氏菌是引起食源性疾病的主要因素之一,包括大肠埃希氏菌O157、O26、O45、O103、O111、O121、O145等血清型。目前,在国内关于大肠埃希氏菌O26的报道及研究相对较少,主要对大肠埃希氏菌O26血清型设计引物并探究优化建立PCR体系,为日常快速检测大肠埃希氏菌O26提供可借鉴的方法。

Escherichia coli is one of the main factors causing foodborne diseases, including Escherichia coli O157, O26, O45, O103, O111, O121, O145 and other serotypes. At present, research and reports on the Escherichia coli O26 are relatively less in the domestic. This paper focuses on the Escherichia coli serotype O26 primer design and optimization on the establishment of PCR system, which may provide references for the daily rapid detection of Escherichia coli O26.