蜘蛛香35%乙醇提取物的肠吸收特性

Intestinal absorption characteristics of 35% ethanol extract from Valeriana jatamansi

目的研究蜘蛛香35%乙醇提取物的肠吸收特性。方法进行大鼠单向肠灌流实验,计算提取物肠灌流液中不同质量浓度(0.5、1.0、1.5 g/mL)新绿原酸、绿原酸、隐绿原酸、异绿原酸B、异绿原酸A、橙皮苷、异绿原酸C吸收参数,考察P-糖蛋白抑制剂(维拉帕米)对提取物吸收的影响。结果除异绿原酸A、橙皮苷外,其他成分K_a、P_(app)值随着其质量浓度增加无明显变化。加入维拉帕米后,各成分(1.0 g/mL)K_a、P_(app)值显著提高(P<0.01)。结论蜘蛛香35%乙醇提取物中7种成分在小肠中呈中等吸收,可能与P-糖蛋白有关。其中,异绿原酸A、橙皮苷吸收机制可能为主动转运,其他成分为自由扩散转运。

AIM To study the intestinal absorption characteristics of 35% ethanol extract from Valeriana jatamansi Jones. METHODS The performance of single-pass intestinal perfusion experiment in rats was succeeded by the calculation of absorption parameters for different concentrations (0.5, 1.0, 1.5 g/ mL) of new chlorogenic acid, chlorogenic acid, hypochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, hesperidin, isochlorogenic acid C in the intestinal perfusate of extract, and the effect of P-glycoprotein inhibitor (verapamil) on extract absorption was investigated. RESULTS The constituents’ Ka and Papp values, except isochlorogenic acid A and hesperidin, did not obviously change with their concentration increase. Yet after the addition of verapamil, significant Ka and Papp values increase was observed (P<0.01). CONCLUSION The moderate small intestinal absorption of seven constituents in 35% ethanol extract from V. jatamansi may be related to P-glycoprotein, among which active transport may be the absorption mechanisms of isochlorogenic acid A and hesperidin, while free-diffusion transport may be those of the other constituents.