诱变剂对芦笋单倍体染色体加倍的比较

Comparative of Mutagenic Agent on Chromosome Doubling of Asparagus Haploid

[目的]建立芦笋单倍体染色体加倍的方法。[方法]以秋水仙碱与Oryzalin为诱变剂(均添加2%DMSO),在离体培养条件下,分别用浸泡法和培养基添加法处理芦笋单倍体材料的茎尖,通过传统的根尖压片法检测染色体数目,比较不同试剂、不同处理方法、不同浓度、不同处理时间下诱导二倍体的效果。[结果]秋水仙碱的处理效果优于试剂Oryzalin;培养基添加法的诱导效果优于浸泡法,在培养基中添加0.2%秋水仙素并处理5 d时诱导效果最佳,染色体加倍效率与成活率分别可达38.3%和50%。[结论]秋水仙碱仍是芦笋单倍体加倍最有效的诱变剂。

[Objective] To establish the method of doubling the haploid chromosome of asparagus.[Method]Under the conditions of in vitro culture,Colchicine and Oryzalin were used as mutants(both added 2 % DMSO). The stem tips of asparagus haploid materials were treated by soaking method and medium addition method, and the number of chromosomes was detected by the traditional root tip pressing method. Induced diploid effect of the different reagents , different treatment methods, different concentrations, different time were compared.[Result] Colchicine was better than the reagent Oryzalin. The induction effect of medium addition method was better than that of immersion method. When 0.2 % colchicine was added to the medium and treated for 5 days, the induction effect was best. The chromosome doubling frequency and survival rate could reach 38.3 % and 50 %, respectively.[Conclusion]Colchicine is still the most effective mutagenic agent for the haploid doubling of asparagus.