竹子内生真菌Fusarium oxysporum次级代谢产物的研究

Study on Secondary Metabolites of Endophytic Fungus Fusarium oxysporum from Bamboo

[目的]对竹子内生真菌尖孢镰刀菌Fusarium oxysporum的次级代谢产物进行分离与鉴定。[方法]运用硅胶柱色谱、Sephadex LH-20凝胶色谱、制备液相色谱等方法进行分离纯化,根据理化性质和波谱技术对化合物的结构进行鉴定。[结果]从该菌的次级代谢产物中分离得到9个化合物,分别鉴定为1-(3, 4-二甲氧基苯基)-乙酮(1)、吲哚-3-乙酸乙酯(2)、吲哚-3-乙酸(3)、吲哚-3-乙酮(4)、吲哚-3-乙酸甲酯(5)、latifolicinin C(6)、白僵菌素(7)、1, 3-diamino-1, 3-dimethylurea(8)和2, 3-丁二醇(9)。[结论]除化合物3、7、9外,其余化合物均为首次从竹子内生真菌Fusarium oxysporum中分离得到。

[Objective] The research aimed to isolate and identificate the secondary metabolites of endophytic fungus Fusarium oxysporum from Bamboo.[Method]The compounds were isolated and purified by the methods of silica gel column chromatography, Sephadex LH-20 gel chromatography, preparative liquid chromatography, etc., and the structures were identified by physicochemical properties and spectral techniques.[Result]Nine compounds were isolated from the endophytic fungus Fusarium oxysporum and their structures were identified as 1-(3,4-Dimethoxyphenyl)-ethanone (1), ethyl indol-3-ylacetate (2), indole-3-acetic acid (3), indol-3-ylethanone (4), methyl indol-3-ylacetate (5), latifolicinin C (6), beauverin (7), 1, 3-diamino-1, 3-dimethylurea (8) and 2,3-Butanediol (9).[Conclusion]Except compound 3, 7 and 9, all the compounds were isolated from endophytic fungus Fusarium oxysporum of Bamboo for the first time.