尼泊尔酸模不同部位提取物及5种主要成分抗炎作用研究

Study on Anti-inflammatory Activities of Different Fractions Extracts and Five Main Ingredients from Rumex nepalensis

[目的]探索尼泊尔酸模(Rumex nepalensis Spreng.,RNS)抗炎的有效部位及活性成分。[方法]采用二甲苯致小鼠耳肿胀和角叉菜胶致大鼠足肿胀模型研究其不同部位和5种主要成分的抗炎作用,并测定大鼠足肿胀组织匀浆中肿瘤坏死因子-α(TNF-α)、大鼠白介素1β(IL-1β)、大鼠血清中超氧化物歧化酶(SOD)以及丙二醛(MDA)的含量。[结果]与模型组相比,30%乙醇部位(RNS2)、50%乙醇部位(RNS3)高剂量组对二甲苯致小鼠耳肿胀和角叉菜胶引起的大鼠足肿胀具有显著抑制作用(P<0.05),表明这2个部位是尼泊尔酸模的有效部位;大黄素、大黄酚和大黄素甲醚可显著降低角叉菜胶致大鼠足肿胀组织中的TNF-α、IL-1β以及血清中MDA的含量(P<0.05)。[结论]尼泊尔酸模具有显著的抗炎作用,其作用机制可能是与抑制脂质氧化终产物MDA,以及调控TNF-α和IL-1β等炎症因子的释放有关。

[Objective]The research aimed to study on anti-inflammatory activities of different fractions and five main ingredients from Rumex nepalensis .[Method]Different fractions and 5 main components were used to investigate the anti-inflammatory effects on auricle edema induced by dimethyl benzene in mice and hand paw swelling induced by carrageenan in rats,and the tumor necrosis factor-α(TNF-α)in rat foot swollen tissue homogenate, rat interleukin-1β(IL-1β), superoxide dismutase (SOD) and malondialdehyde (MDA) in rat serum were measured.[Result]Compared to the model group,the RNS2 and RNS3 could relieve swollen auricle of mice by dimethyl benzene and hand paw swelling ratsby carrageenan( P <0.05), indicating that these two parts were the main anti-inflammatory effective fractions. Meanwhile, emodin, chrysophanol and physcion showed anti-inflammatory effects on reducing the level of TNF-α, IL-1β and MDA ( P <0.05).[Conclusion]RNS has significant anti-inflammatory effects. The mechanism may be related to the inhibition of MDA levels and the regulation of the release of inflammatory factors such as TNF-α and IL-1β.