染料木黄酮通过ERK及JNK通路抑制人非小细胞肺癌PC14细胞增殖

Genistein Inhibits the Proliferation of Human Lung Cancer PC14 Cells through the ERK and JNK Pathways

目的观察染料木黄酮对人非小细胞肺癌PC14细胞增殖的影响,并探讨其作用机制。方法 MTT法及集落形成实验检测细胞增殖;蛋白质免疫印迹检测蛋白表达水平。结果染料木黄酮能显著抑制PC14细胞的增殖,其抗增殖活性具有浓度和时间依赖性;MAPK家族特异性抑制剂PD98059、SB203580及SP600125均可显著抑制PC14细胞增殖;染料木黄酮可剂量依赖性地抑制ERK及JNK的磷酸化。结论染料木黄酮可抑制人非小细胞肺癌PC14细胞的增殖,其机制可能与其抑制ERK及JNK的活化有关。

Objective To investigate the effect of genistein on the proliferation of human lung cancer PC14 cells and the underlying mechanisms. Methods Cell proliferation was examined using the MTT and colony formation assays. Western blotting was used to analyze protein expression levels. Results Genistein significantly inhibited the proliferation of PC14 cells in a concentration and time dependent manner. PD98059,SB203580,and SP100625,three specific inhibitors of the MAPK pathway,significantly inhibited the proliferation of PC14 cells. Moreover,genistein inhibited the phosphorylation of ERK and JNK in a dose dependent manner. Conclusion Genistein can inhibit the proliferation of PC14 cells,which may be related to its inhibitory effect on ERK and JNK activation.