RP-HPLC法测定17批不同产地苍术中苍术素的含量

CONTENT DETERMINATION OF ATRACTYLODIN IN 17 BATCHES OF ATRACTYLODES LANCEA(THUNB) DC FROM DIFFERENT HABITATS BY RP-HPLC

目的采用RP-HPLC法建立茅苍术中苍术素含量的测定方法,并对17批不同产地茅苍术药材中苍术素含量进行分析,为评价茅苍术药材质量提供参考。方法采用Agilent Eclipse XDB-C18(250 mm×4.6 mm,5μm)色谱柱;以乙腈-水(65∶35,V/V)为流动相;流速1.0 mL·min^(-1);柱温25℃;检测波长340 nm。结果方法学考察表明:苍术素浓度在0.705～42.3μg·mL^(-1)的范围内与峰面积呈良好的线形关系(r=0.9997),回归方程为Y=243188X-31.843,平均加样回收率为99.15%,RSD为1.06%(n=6),建立的方法重复性、耐用性、准确度良好。结论对17批样品进行分析,结果显示不同产地茅苍术中苍术素的含量差异较大,河南省方城县和嵩县的茅苍术中苍术素的含量明显高于其他产地,本实验为茅苍术药材的质量评价和控制提供了一定的科学依据。

Object In order to evaluate the quality of Atractylodes lancea,RP-HPLC method was established to determinate the content of atractylodin in 17 batches of Atraxtylodi Rhizoma from different habitats.Methods The determination was performed on Agilent Eclipse XDB-C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of acetonitrile-water(65∶35,V/V) at the flow rate of 1 mL·min^-1.The detection wavelength was 340 nm,the colum temperature was 25 ℃.Results The methodological evaluation showed that there was good linear relationship for atractylodin in range of 0.705~42.3 μg·mL^-1(r=0.9997),the regression equation was Y=243188 X-31.843,the average recovery rate was 99.15%,RSD=1.06%(n=6).The developed method had good reproducibility,robustness and accuracy.Conclusion The analysis of 17 batches of samples exhibited that there were significant different among contents of atractylodin in Atractylodes lancea from different habitats.The results showed that contents of atractylodin in Atractylodes lancea from Fangcheng county and Songshan county of Henan province were apparently higher than that of other areas.This experiment provides a scientific basis for evaluation and control of the quality of Atractylodes lancea.