红树莓籽原花青素提取及其结合胆酸盐能力评价

Extraction of Proanthocyanidin from Red Raspberry Seed and Evaluation of Its Capacities of Binding Bile Salts

通过响应面法优化超声波-酶法辅助提取红树莓籽原花青素工艺,并对其结合胆酸盐能力进行评价。以原花青素得率为响应值,利用Box-Behnken设计对影响原花青素得率的主要因素进行优化,确定最优提取条件为:料液比1∶31(g/mL)、酶添加量0.8%、超声功率190 W、提取时间39 min,此条件下原花青素得率达16.42 mg/g。红树莓籽原花青素对胆酸盐结合速度较快,30 min内达到吸附平衡;红树莓籽原花青素结合牛磺胆酸钠能力最强,对胆酸钠、甘氨胆酸钠和牛磺胆酸钠结合率相当于同剂量考来烯胺结合率的54.50%、71.79%和72.34%,说明红树莓籽原花青素具有良好的体外结合胆酸盐能力。

The ultrasound-enzyme assisted on red raspberry seed proanthocyanidins was optimized by response surface methodology, also evaluated its ability of binding bile salts in this research. Based on Box-Benhnken central combination design, extraction rate of proanthocyanidins was taken as index, effects of four crucial factors on extraction rate of proanthocyanidins were investigated by response surface analysis. The optimum extraction conditions that provided the maximum extraction rate (16.42 mg/g) of proanthocyanidins were determined as follows: ratio of solid to liquid, 1∶31(g/mL);enzyme dosage, 0.8 %;ultrasound power, 190 W;extraction time, 39 min. At the beginning of the reaction, the proanthocyanidins combines three cholic acid salt was faster and the adsorption equilibrium was reached in 30 min. Sodium taurocholate was better than sodium glycocholate and sodium cholate in their binding ability to red raspberry seed procyanidins. Compared with cholestyramine, its combination with the sodium cholate, sodium glycocholate and sodium taurocholate values were, 54.50 %, 71.79 %, 72.34 %. Thus red raspberry seed proanthocyanidins have good binding capacity with bile salts in vitro.