膀胱癌组织MFN2、Bmi-1蛋白表达及临床意义分析

Expression of MFN2 and Bmi-1 protein in bladder cancer and its clinical significance

目的探讨膀胱癌组织中线粒体融合蛋白2(MFN2)、Bmi-1蛋白表达情况及临床意义。方法选取膀胱癌组织标本80例,同时另选取正常膀胱组织标本80例作为对照。采用免疫组化染色法检测并比较膀胱癌组织和正常膀胱组织中MFN2、Bmi-1蛋白表达情况,分析MFN2、Bmi-1蛋白表达与膀胱癌患者临床特征的关系,以及膀胱癌组织中MFN2蛋白与Bmi-1蛋白表达的相关性。结果膀胱癌组织中MFN2和Bmi-1蛋白的阳性表达率分别为52.50%和61.25%,高于正常膀胱组织的12.50%和17.50%(P﹤0.05);TNM分期为Ⅰ～Ⅱ期的患者膀胱癌组织中MFN2蛋白的阳性表达率为67.27%,明显高于TNM分期为Ⅲ期患者的20.00%(P﹤0.01);肿瘤直径﹥3 cm、TNM分期为Ⅲ期的患者膀胱癌组织中Bmi-1蛋白的阳性表达率分别为96.15%和88.00%,均明显高于肿瘤直径≤3 cm、TNM分期为Ⅰ～Ⅱ期患者的44.44%、49.09%(P﹤0.01);膀胱癌组织中MFN2蛋白与Bmi-1蛋白的表达情况呈负相关(r=-0.602,P﹤0.01)。结论膀胱癌组织中MFN2、Bmi-1蛋白表达水平较高;MFN2蛋白阳性表达情况与TNM分期有关,Bmi-1蛋白阳性表达情况与肿瘤直径、TNM分期有关;MFN2蛋白与Bmi-1蛋白的表达情况呈负相关。

Objective To investigate the expression and clinical significance of mitofusion 2 (MFN2) and Bmi-1 protein in bladder cancer and the clinical significance. Method Eighty cases of bladder cancer and another 80 cases with normal bladder tissues were included in the study. The expression of MFN2 and Bmi-1 protein in these subjects were detected by immunohistochemical staining to investigate the relationship with the clinical characteristics of bladder cancer patients, and the correlation between the expression of MFN2 protein and Bmi-1 protein in bladder cancer tissues. Result The positive rates of MFN 2 and Bmi-1 protein in bladder cancer tissues were 52.50% and 61.25% respectively, which were significantly higher than those in normal bladder tissues at 12.50% and 17.50%(P<0.05);the positive expression rate of MFN2 protein in TNM stage Ⅰ~Ⅱ patients was 67.27%, and was significantly higher than that in patients with TNM stage Ⅲ disease at 20.00%(P<0.01);the positive rates of Bmi-1 protein in patients with tumor diameter > 3 cm and TNM stage Ⅲ were 96.15% and 88.00% respectively, which were significantly higher than those in patients with tumor diameter ≤3 cm and stage Ⅰ~Ⅱ disease at 44.44% and 49.09%, respectively (P<0.01);there was a negative correlation between the expression of MFN2 protein and Bmi-1 protein in bladder cancer tissues (r=-0.602, P<0.01). Conclusion The expression of MFN2 and Bmi-1 protein in bladder cancer tissues are high, besides, the positive expression of MFN2 protein is correlated with the TNM staging, and that of Bmi-1 protein is associated with tumor diameter and TNM staging, additionally, MFN2 protein is negatively correlated with Bmi-1 protein.