特殊生境微生物培养技术研究进展

Research Progress of Microbial Culture Techniques in Special Habitats

针对细菌耐药性日益增强,抗生素匮乏、特殊生境微生物药用资源开发不足等现状,对特殊生境微生物培养技术进行综述;总结制约特殊生境微生物生长的因素,概述设计开发新型的微生物培养基、改善微生物培养条件等方面研究进展,重点综述一些新颖独特的微生物培养技术和方法,包括群体培养与共培养、高通量培养技术、原位培养、细胞微囊包埋技术、分子生物学方法等;指出经典培养方法的优化使特殊生境微生物的可培养性显著改善,基于分子生物学方法的研究结果不仅丰富了微生物资源库,而且为今后特殊生境微生物分离与培养指出了明确方向。

In view of the increasingly severe drug resistance,the lack of antibiotics,and the inadequacy in the development of medicinal resources for special habitats,a review was made of microbial culture techniques in special habitats.The factors restricting the growth of microorganisms in special habitats were summarized,the research and development of the design and development of new microbial culture media were outlined,and the conditions of microbial culture were improved.Some of new and unique microbial culture techniques and methods were highlighted,including population culture and co-culture,high-throughput culture,in-situ culture,cell microencapsulation technology,and molecular biology methods.It was pointed out that the optimization of the classical culture method made the microorganism more culturable in special habitats.The results of the study based on the molecular biology method not only enriched the microbial resource pool,but also pointed out a clear direction for the isolation and culture of the special habitats in future.