外源性的脉冲电磁场刺激对于糖尿病皮肤创伤愈合作用的实验研究

The promotional effects of exogenous stimulation with pulsed electromagnetic fields on skin wound healing in diabetic rats

目的:阐明通过外源性施加脉冲电磁场刺激对于糖尿病大鼠皮肤创伤修复的作用效果,为其未来的更科学临床应用提供实验依据。方法:使用腹腔注射链脲佐菌素对4月龄雄性大鼠构建糖尿病动物模型,取48只糖尿病大鼠随机等分为糖尿病组(DM)和糖尿病协同脉冲电磁场刺激组(DM+PEMF),另取24只作为对照组(Control)。随后在3组大鼠背部通过手术构建皮肤损伤模型(直径2 cm的圆形损伤),随后对DM+PEMF组大鼠施加脉冲电磁场刺激,分别在手术后的第5、12、19天每组处死6只大鼠,进行血糖、伤口闭合率、伤口愈合时间、组织抗张强度的检测分析。结果:脉冲电磁场在术后的第5、12、19天糖尿病伤口的闭合率显著增加,糖尿病创伤的愈合时间缩短,并且在术后的第5、12、19天糖尿病伤口外周组织的力学抗张强度均提升,差异均具有统计学意义(P<0.01)。结论:低强度脉冲电磁场刺激能够显著加速糖尿病伤口的愈合进程,提高糖尿病软组织的损伤修复能力。本研究有望为脉冲电磁场对糖尿病创伤的更科学、合理的临床应用提供有力的实验依据。

Objective:The current study aims to identify the effects of exogenous application of pulsed electromagnetic fields on skin wound healing in diabetic rats,and thus provides experimental evidence for its more scientific clinical application in the future.Methods:The diabetic animal models were established via intraperitoneal injection of streptozotocin in 4-month-old male rats.Fourth eight rats were randomly assigned into the diabetes group(DM)and diabetes coupled with pulsed electromagnetic fields(DM+PEMF)group.Another 24 normal rats were used as the blank control group(Control).Then,all the rats in the three groups were subjected to dorsal surgery for the establishment of soft tissue wound model(circular wound with the diameter of 2 cm).Then,the rats in the DM+PEMF group were subjected to pulsed electromagnetic fields stimulation.Then,6 rats in each group were sacrificed at Day 5,12 and 19 post surgery.The glucose levels,wound closure,wound healing time and tissue tensile strength were examined and analyzed.Results:Pulsed electromagnetic fields significantly increased the wound closure rate in diabetic rats at Day 5,12 and 19 post surgery(P<0.01),decreased overall wound healing period in diabetic rats(P<0.01),and also enhanced tissue tensile strength in diabetic rats at Day 5,12 and 19 post surgery(P<0.01).Conclusion:Low-intensity pulsed electromagnetic fields can significantly accelerate diabetic wound healing process,and also improve diabetic tissue repair capacity.This study may be helpful for providing more scientific and reasonable experimental evidence for the treatment of pulsed electromagnetic fields on diabetic wound healing in clinics.