健康与发病香蕉植株内生细菌菌群差异

Endophytic bacterial community between healthy and diseased banana plants

[目的]本研究通过探究健康和发病‘巴西香牙蕉’植株根、茎、叶内生细菌群落结构差异,为生防菌筛选和香蕉枯萎病的防控工作提供参考。[方法]通过MiSeq高通量测序细菌16S核糖体RNA分析香蕉枯萎病发病蕉园健康和发病‘巴西香牙蕉’(Musa AAA cavendish cv. Brazil)植株根、茎、叶内生细菌群落结构与多样性。[结果]健康‘巴西香牙蕉’植株根内生细菌菌群多样性和丰富度指数最高分别是6.11和671,其次为叶,分别是5.49和370,最后是茎,分别是2.50和237;发病‘巴西香牙蕉’植株的根内生细菌菌群多样性和丰富度指数分别为6.86和1 226,茎部内生细菌菌群多样性和丰富度指数分别为5.08和731,从数值上看总体高于健康香蕉植株处理,其中茎部差异显著;健康与发病‘巴西香牙蕉’植株内生细菌群落结构与组成差异显著,其中代尔夫特菌属(Delftia)、芽胞杆菌属(Bacillus)、海洋放线菌属(Salinispora)、不黏柄菌属(Asticcacaulis)和动胶菌属(Zoogloea)是抽蕾后健康‘巴西香牙蕉’植株的特有内生细菌属。[结论]‘巴西香牙蕉’植株对菌群吸收具有选择性,且根、茎、叶对微生物的选择性存在差异。‘巴西香牙蕉’内生细菌群落结构、多样性、丰富度与香蕉植株健康水平密切相关,病原菌入侵降低了香蕉植株对外源菌的选择性。

[Objectives] Fusarium wilt of banana is devastating banana plantations worldwide.In order to provide reference for biocontrol bacteria isolation and disease control,the endophytic bacterial communities in different parts of healthy and diseased banana(Musa AAA cavendish cv.Brazil)plants were studied.[Methods] The endophytic bacterial communities in different parts of healthy and diseased banana plants were profiled through MiSeq high-throughput sequencing of the bacterial 16S rRNA amplicons.[Results] The endophytic bacterial community Shannon diversity and Chao1 richness index in roots of healthy banana plants were 6.11 and 671,followed by leaves which were 5.49 and 370,and stems which were 2.50 and 237.The Shannon diversity and Chao1 richness index in root of diseased banana plants were 6.86 and 1 226,followed by stems which were 5.08 and 731,which were higher than those of healthy banana plants though the difference of Shannon diversity of root was insignificant.The endophytic bacterial community patterns were significantly different between the healthy and diseased banana plants.Delftia,Bacillus,Salinispora,Asticcacaulis and Zoogloea were indicator genera in healthy banana plants which were not detected in the diseased banana plants after buds.[Conclusions] The banana plants selectively absorb microbiome from the environment that different parts of banana plants had different endophytic community.The endophytic bacterial community composition and the diversity of banana plants were closely related to their health levels.The invasion of pathogen reduced the prevention level of banana plants to the microbial community outside of the plants.