那可丁对人肺癌细胞A549生物学功能的影响

Effect of Noscapine on the Biological Function of A549 Cells

目的探讨那可丁对人肺癌细胞系A549细胞生物学功能的影响及其作用机制。方法使用那可丁(10μmol/L和20μmol/L)作用于A549细胞,通过MTT观察那可丁对细胞增殖的影响;通过Western blotting和实时PCR检测那可丁作用于A549细胞后CyclinD1、 BCL-2、MMP2蛋白和mRNA的表达;Hoechst 33258染色检测那可丁对A549细胞凋亡的影响;Transwell检测那可丁对细胞转移的影响。结果 MTT检测发现10、20μmol/L那可丁分别作用于A549细胞24 h后对细胞增殖抑制率分别为(32.98±1.09)%和(49.56±3.98)%,差异有统计学意义(P <0.05)。Hoechst 33258染色显示那可丁促进细胞凋亡,Transwell实验显示那可丁抑制A549细胞转移。Western blotting和实时PCR结果显示那可丁抑制A549细胞中Cyclin D1、BCL-2和MMP2的表达。结论那可丁能够抑制人肺癌细胞系A549细胞生长及转移、促进A549细胞凋亡,可以作为治疗肺癌的潜在药物。

Objective To explore the effect of noscapine on the biological function of A549 cells and its underlying mechanism. Methods Concentrations of 10 μmol/L and 20 μmol/L of noscapine were used on the A549 cells. The effect of noscapine on their proliferation was observed via the MTT assay. Western blotting and real time PCR were used to detect the effects on Cyclin D1,BCL-2,and MMP2. The Hoechst 33258 staining assay was used to observe the effects of noscapine on the apoptosis of A549 cells. The metastasis of A549 cells was detected by using the Transwell assay. Results The MTT assay showed that the rate of inhibition of cell proliferation after treatment with 10 μmol/L and 20 μmol/L noscapine were( 32.98±1.09)% and( 49.56±3.98)%,with a significant difference between the values( P < 0.05). Hoechst 33258 staining evinced that noscapine promoted apoptosis,while the Transwell assay displayed that noscapine inhibited A549 cell metastasis. Western blotting and real time PCR indicated that the expression of Cyclin D1,BCL-2,and MMP2 in A549 cells was inhibited by noscapine. Conclusion Noscapine can inhibit the growth and metastasis of the lung cancer cell line A549 and promote the apoptosis of A549 cells. In conclusion,noscapine can potentially be used as a chemotherapeutic drug for lung cancer.