宫颈组织中Stat5b表达与宫颈病变的关系

Relationship between Stat5b Expression in Cervical Tissues and Lesions

目的研究宫颈病变组织中信号转导和转录激活因子Stat5b及其磷酸化形式p-Stat5b的表达情况及其与不同程度宫颈病变之间的关系,探讨Stat5b在宫颈病变发生、发展中的作用。方法以正常宫颈组织、低度鳞状上皮内病变(LSIL)、高度鳞状上皮内病变(HSIL)、宫颈癌组织共80例标本为研究对象,采用免疫组织化学染色评估Stat5b以及p-Stat5b的表达水平,分析其与宫颈病变程度的相关性。结果 Stat5b表达水平随宫颈病变的进展而升高,呈正相关。Stat5b在正常宫颈组织、LSIL、HSIL、宫颈癌中高表达比例分别为15.0%、0.0%、50.0%、80.0%,差异有统计学意义(P <0.05)。相反,p-Stat5b表达与宫颈病变的进展呈负相关,在正常宫颈组织、LSIL、HSIL、宫颈癌中高表达比例分别为80.0%、45.0%、5.0%、0.0%,差异有统计学意义(P <0.05)。结论 Stat5b在不同程度宫颈病变中的表达有差异,表明Stat5b信号分子可能与宫颈病变的发展有关。

Objective To investigate the expression of signal transducer and activator of transcription 5 b(Stat5 b)and its phosphorylation(p-Stat5 b)in cervical lesions,its relationship with different degrees of cervical lesions,and its role in the pathogenesis and development of cervical lesions.Methods We obtained 80 specimens,including normal cervical tissues,low-grade squamous intraepithelial lesions(LSIL),high-grade squamous intraepithelial lesions(HSIL),and cervical cancer tissues.To analyze the correlation between Stat5 b and the degree of cervical lesions,immunohistochemical staining was performed to detect Stat5 b and p-Stat5 b expression.Results Increased Stat5 b expression had a positive correlation with the development of cervical lesions.The percentages of Stat5 b expressed in normal tissues,LSIL,HSIL,and cervical cancer tissues were 15.0%,0.0%,50.0%,and 80.0%,respectively,with significant differences among the groups(P<0.05).Conversely,the expression of p-Stat5 b had a negative correlation with the development of cervical lesions.Expression of p-Stat5 b in normal tissues,LSIL,HSIL,and cervical cancer tissues was 80.0%,45.0%,5.0%,and 0.0%,respectively,with statistically significant differences among the groups(P<0.05).Conclusion The expression of Stat5 b is significantly different in different degrees of cervical lesions,suggesting that Stat5 b signaling molecules may be involved in the development of cervical lesions.